Haplotypes of the HLA-G 3′ untranslated region respond to endogenous factors of HLA-G+ and HLA-G- cell lines differentially

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dc.contributor.authorPoras, Isabelle
dc.contributor.authorYaghi, Layale
dc.contributor.authorMartelli-Palomino, Gustavo
dc.contributor.authorMendes, Celso T.
dc.contributor.authorMuniz, Yara Costa Netto
dc.contributor.authorCagnin, Natalia F.
dc.contributor.authorDe Almeida, Bibiana Sgorla
dc.contributor.authorCastelli, Erick C. [UNESP]
dc.contributor.authorCarosella, Edgardo D.
dc.contributor.authorDonadi, Eduardo A.
dc.contributor.authorMoreau, Philippe
dc.contributor.institutionHôpital Saint-Louis
dc.contributor.institutionSchool of Medicine
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:23:20Z
dc.date.available2018-12-11T17:23:20Z
dc.date.issued2017-01-01
dc.description.abstractThe immune checkpoint HLA-G prevents maternal rejection of the fetus and contributes in cancer invasion and acceptance of allografts. The 5′ and 3′ regulatory regions of the HLA-G gene are polymorphic and balancing selection probably maintains this variability. It is proposed that nucleotide variations may affect the level of HLA-G expression. To investigate this issue we aimed to analyze how haplotypes of the 3′ untranslated region (3′UTR) with highest worldwide frequencies, namely UTR-1, UTR-2, UTR-3, UTR-4, UTR-5, UTR-18 and UTR-7, impact the expression of a luciferase reporter gene in vitro. Experiments performed with the HLA-G positive cell lines JEG-3 (choricarcinoma) and FON (melanoma), and with the HLA-G negative cell lines M8 (melanoma) and U251MG (glioblastoma) showed that the HLA-G 3′UTR polymorphism influences the response to endogenous cellular factors and may vary according to the cell type. UTR-5 and UTR-7 impact the activity of luciferase the most whereas UTR-2, UTR-3, UTR-4, and UTR-18 have intermediate impact, and UTR-1 has the lowest impact. These results corroborate the previous associations between amounts of plasma sHLA-G levels and 3′UTR haplotypes in healthy individuals and reinforce that 3′UTR typing may be a predictor of the genetic predisposition of an individual to express different levels of HLA-G.en
dc.description.affiliationCommissariat à l'Energie Atomique et aux Energies Alternatives Institut des Maladies Emergentes et des Thérapies Innovantes Service de Recherches en Hémato-Immunologie Hôpital Saint-Louis
dc.description.affiliationUniversité Paris-Diderot Sorbonne Paris-Cité UMR-E5 Institut Universitaire D'Hématologie Hôpital Saint-Louis
dc.description.affiliationLebanese University School of Medicine
dc.description.affiliationPrograma de Pósgraduação em Ciências da Saúde Centro de Ciências da Saúde Universidade Federal do Rio Grande do Norte
dc.description.affiliationDepartamento de Química Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartamento de Biologia Celular Embriologia e Genética Centro de Ciências Biológicas Universidade Federal de Santa Catarina
dc.description.affiliationDepartment of Genetics Ribeirão Preto Medical School University of São Paulo
dc.description.affiliationDivisão de Imunologia Clínica Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo
dc.description.affiliationDepartment of Pathology School of Medicine Universidade Estadual Paulista Unesp
dc.description.affiliationUnespDepartment of Pathology School of Medicine Universidade Estadual Paulista Unesp
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 014747/2013-8
dc.description.sponsorshipIdFAPESP: 2014/18730-9
dc.description.sponsorshipIdCNPq: 406594/2013-9
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0169032
dc.identifier.citationPLoS ONE, v. 12, n. 1, 2017.
dc.identifier.doi10.1371/journal.pone.0169032
dc.identifier.file2-s2.0-85008192046.pdf
dc.identifier.issn1932-6203
dc.identifier.scopus2-s2.0-85008192046
dc.identifier.urihttp://hdl.handle.net/11449/176972
dc.language.isoeng
dc.relation.ispartofPLoS ONE
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleHaplotypes of the HLA-G 3′ untranslated region respond to endogenous factors of HLA-G+ and HLA-G- cell lines differentiallyen
dc.typeArtigo

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Artigos - Microbiologia e Imunologia - IBB