Impacts of in vivo and in vitro exposures to tamoxifen: Comparative effects on human cells and marine organisms

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dc.contributor.authorFonseca, T. G. [UNESP]
dc.contributor.authorCarriço, T.
dc.contributor.authorFernandes, E.
dc.contributor.authorAbessa, D. M.S. [UNESP]
dc.contributor.authorTavares, A.
dc.contributor.authorBebianno, M. J.
dc.contributor.institutionUniversidade do Algarve
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-06T16:31:47Z
dc.date.available2019-10-06T16:31:47Z
dc.date.issued2019-08-01
dc.description.abstractTamoxifen (TAM) is a first generation-SERM administered for hormone receptor-positive (HER+) breast cancer in both pre- and post-menopausal patients and may undergo metabolic activation in organisms that share similar receptors and thus face comparable mechanisms of response. The present study aimed to assess whether environmental trace concentrations of TAM are bioavailable to the filter feeder M. galloprovincialis (100 ng L−1) and to the deposit feeder N. diversicolor (0.5, 10, 25 and 100 ng L−1) after 14 days of exposure. Behavioural impairment (burrowing kinetic), neurotoxicity (AChE activity), endocrine disruption by alkali-labile phosphate (ALP) content, oxidative stress (SOD, CAT, GPXs activities), biotransformation (GST activity), oxidative damage (LPO) and genotoxicity (DNA damage) were assessed. Moreover, this study also pertained to compare TAM cytotoxicity effects to mussels and targeted human (i.e. immortalized retinal pigment epithelium – RPE; and human transformed endothelial cells – HeLa) cell lines, in a range of concentrations from 0.5 ng L−1 to 50 μg L−1. In polychaetes N. diversicolor, TAM exerted remarkable oxidative stress and damage at the lowest concentration (0.5 ng L−1), whereas significant genotoxicity was reported at the highest exposure level (100 ng L−1). In mussels M. galloprovincialis, 100 ng L−1 TAM caused endocrine disruption in males, neurotoxicity, and an induction in GST activity and LPO byproducts in gills, corroborating in genotoxicity over the exposure days. Although cytotoxicity assays conducted with mussel haemocytes following in vivo exposure was not effective, in vitro exposure showed to be a feasible alternative, with comparable sensitivity to human cell line (HeLa).en
dc.description.affiliationCIMA Centro de Investigação Marinha e Ambiental Universidade do Algarve, Campus Gambelas
dc.description.affiliationNEPEA Núcleo de Estudos em Poluição e Ecotoxicologia Aquática Universidade Estadual Paulista (UNESP), Campus do Litoral Paulista
dc.description.affiliationDepartamento de Ciências Biomédicas e Medicina Universidade do Algarve, Campus Gambelas
dc.description.affiliationUnespNEPEA Núcleo de Estudos em Poluição e Ecotoxicologia Aquática Universidade Estadual Paulista (UNESP), Campus do Litoral Paulista
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 202360/2014-8
dc.format.extent256-272
dc.identifierhttp://dx.doi.org/10.1016/j.envint.2019.05.014
dc.identifier.citationEnvironment International, v. 129, p. 256-272.
dc.identifier.doi10.1016/j.envint.2019.05.014
dc.identifier.issn1873-6750
dc.identifier.issn0160-4120
dc.identifier.scopus2-s2.0-85066108349
dc.identifier.urihttp://hdl.handle.net/11449/189162
dc.language.isoeng
dc.relation.ispartofEnvironment International
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomarkers
dc.subjectCytotoxicity
dc.subjectHuman cells
dc.subjectMarine organisms
dc.subjectTamoxifen
dc.titleImpacts of in vivo and in vitro exposures to tamoxifen: Comparative effects on human cells and marine organismsen
dc.typeArtigo

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