Purification and Characterization of the alpha-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756

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dc.contributor.authorAzevedo Carvalho, Ana Flavia [UNESP]
dc.contributor.authorBoscolo, Mauricio [UNESP]
dc.contributor.authorda Silva, Roberto [UNESP]
dc.contributor.authorFerreira, Henrique [UNESP]
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:00:48Z
dc.date.available2014-05-20T14:00:48Z
dc.date.issued2010-08-01
dc.description.abstractAn alpha-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose 12 columns. The apparent molecular mass of the enzyme was 83 kDa as determined in gel electrophoresis. Maximum activity was observed at pH 4.5 at 70 degrees C. Enzyme showed stability stable in the pH range of 3.0-9.0 and lost 40% of its initial activity at the temperatures of 40, 50, and 60 C. In the presence of ions Na(+), Ba(2+), Co(2+), Ni(2+), Mg(2+), Mn(2+), Al(3+), Zn(2+), Ca(2+) this enzyme maintained 90-105% of its maximum activity and was inhibited by Cr(3+), Ag(+), and Hg(2+). The enzyme showed a transglycosylation property, by the release of oligosaccharides after 3 h of incubation with maltose, and specificity for short maltooligosaccharides and alpha-PNPG. The K(m) measured for the a-glucosidase was 0.07 mu M, with a V(max) of 318.0 mu mol/min/mg.en
dc.description.affiliationSão Paulo State Univ Unesp, Lab Bichem & Appl Microbiol, BR-15054000 Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespSão Paulo State Univ Unesp, Lab Bichem & Appl Microbiol, BR-15054000 Sao Jose do Rio Preto, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent452-459
dc.identifierhttp://dx.doi.org/10.1007/s12275-010-9319-2
dc.identifier.citationJournal of Microbiology. Seoul: Microbiological Society Korea, v. 48, n. 4, p. 452-459, 2010.
dc.identifier.doi10.1007/s12275-010-9319-2
dc.identifier.issn1225-8873
dc.identifier.lattes8880074921989984
dc.identifier.lattes9424175688206545
dc.identifier.lattes7091241742851920
dc.identifier.urihttp://hdl.handle.net/11449/21476
dc.identifier.wosWOS:000281516800007
dc.language.isoeng
dc.publisherMicrobiological Society Korea
dc.relation.ispartofJournal of Microbiology
dc.relation.ispartofjcr2.319
dc.relation.ispartofsjr0,911
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectalpha-glucosidaseen
dc.subjectT. aurantiacusen
dc.subjecttermostable enzymeen
dc.subjecttransglycosylation reactionen
dc.subjectPurificationen
dc.titlePurification and Characterization of the alpha-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756en
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderMicrobiological Society Korea
unesp.author.lattes8880074921989984
unesp.author.lattes9424175688206545
unesp.author.lattes7091241742851920
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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