Possible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinoma

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dc.contributor.authorHassumi-Fukasawa, Marcela K.
dc.contributor.authorMiranda-Camargo, Fabiana A.
dc.contributor.authorGuimaraes, Marcia C. M.
dc.contributor.authorSimoes, Renata T.
dc.contributor.authorDonadi, Eduardo A.
dc.contributor.authorSoares, Christiane P.
dc.contributor.authorSoares, Edson G. [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHosp Santa Casa Belo Horizonte
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:31:11Z
dc.date.available2014-05-20T15:31:11Z
dc.date.issued2012-07-01
dc.description.abstractLaryngeal squamous cell carcinoma is one of the most common malignant neoplasms of the head and neck. In Brazil, laryngeal tumors represent 2% of all cancers and are associated with approximately 3,000 deaths annually. Human papillomavirus (HPV) has been reported to play an important role in the etiology of laryngeal cancer. The aim of the present study was to evaluate the expression of p53, p27, and Mdm2 in laryngeal carcinomas. Sixty-three larynx biopsies were selected for the study, including 9 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastasis and 27 laryngeal carcinomas with metastasis. Twenty-seven cervical lymph nodes from patients with metastatic lesions were also evaluated. The expression levels of p53, p27, and Mdm2 were assessed by immunohistochemistry using a computer-assisted system. HPV detection and typing were performed using PCR, and the HPV types that were evaluated included HPV 6, 11, 16, 18, 31 and 33. Out of 63 patients, 53 (84.1%) were positive for beta-globin (internal control), and 10 (15.9%) were beta-globin negative and therefore excluded from the evaluation. Thus, 7 (13.2%) out of 53 patients were HPV positive, and 46 (86.8%) out of 53 patients were HPV negative. Statistically significant differences (p < 0.05) in Mdm2 expression levels were observed in the in situ laryngeal carcinoma samples compared with the laryngeal carcinoma samples with metastasis. No statistically significant differences (p > 0.05) in either p53 or p27 expression levels were detected. These findings suggest that Mdm2 may be associated with the invasiveness and aggressiveness of laryngeal carcinomas.en
dc.description.affiliationUniv São Paulo, Dept Pathol, Fac Med Ribeirao Preto, BR-14049900 Ribeirao Preto, SP, Brazil
dc.description.affiliationHosp Santa Casa Belo Horizonte, Belo Horizonte, MG, Brazil
dc.description.affiliationUniv São Paulo, Dept Med, Fac Med Ribeirao Preto, Div Clin Immunol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Sch Pharmaceut Sci, Dept Clin Anal, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sch Pharmaceut Sci, Dept Clin Anal, São Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1795-1804
dc.identifierhttp://dx.doi.org/10.1007/s00405-012-1937-3
dc.identifier.citationEuropean Archives of Oto-rhino-laryngology. New York: Springer, v. 269, n. 7, p. 1795-1804, 2012.
dc.identifier.doi10.1007/s00405-012-1937-3
dc.identifier.issn0937-4477
dc.identifier.lattes1768025290373669
dc.identifier.orcid0000-0003-1740-7360
dc.identifier.urihttp://hdl.handle.net/11449/40397
dc.identifier.wosWOS:000304700600011
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofEuropean Archives of Oto-rhino-laryngology
dc.relation.ispartofjcr1.546
dc.relation.ispartofsjr0,800
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSquamous cell carcinomaen
dc.subjectLarynxen
dc.subjectHPVen
dc.subjectp53en
dc.subjectp27en
dc.subjectMdm2en
dc.titlePossible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinomaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes1768025290373669[6]
unesp.author.orcid0000-0002-9457-9601[5]
unesp.author.orcid0000-0003-1740-7360[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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