Population Dynamics and Growth Rates of Endosymbionts During Diaphorina citri (Hemiptera, Liviidae) Ontogeny

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dc.contributor.authorAlda Dossi, Fabio Cleisto
dc.contributor.authorSilva, Edney Pereira da [UNESP]
dc.contributor.authorConsoli, Fernando Luis
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:52:56Z
dc.date.available2015-03-18T15:52:56Z
dc.date.issued2014-11-01
dc.description.abstractThe infection density of symbionts is among the major parameters to understand their biological effects in host-endosymbionts interactions. Diaphorina citri harbors two bacteriome-associated bacterial endosymbionts (Candidatus Carsonella ruddii and Candidatus Profftella armatura), besides the intracellular reproductive parasite Wolbachia. In this study, the density dynamics of the three endosymbionts associated with the psyllid D. citri was investigated by real-time quantitative PCR (qPCR) at different developmental stages. Bacterial density was estimated by assessing the copy number of the 16S rRNA gene for Carsonella and Profftella, and of the ftsZ gene for Wolbachia. Analysis revealed a continuous growth of the symbionts during host development. Symbiont growth and rate curves were estimated by the Gompertz equation, which indicated a negative correlation between the degree of symbiont-host specialization and the time to achieve the maximum growth rate (t*). Carsonella densities were significantly lower than those of Profftella at all host developmental stages analyzed, even though they both displayed a similar trend. The growth rates of Wolbachia were similar to those of Carsonella, but Wolbachia was not as abundant. Adult males displayed higher symbiont densities than females. However, females showed a much more pronounced increase in symbiont density as they aged if compared to males, regardless of the incorporation of symbionts into female oocytes and egg laying. The increased density of endosymbionts in aged adults differs from the usual decrease observed during host aging in other insect-symbiont systems.en
dc.description.affiliationUniv Sao Paulo, Coll Agr Luiz de Queiroz, Dept Entomol & Acarol, Insect Interact Lab, BR-13418900 Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ, Coll Agr & Veterinarian Sci, Dept Anim Sci, Avian Sci Lab, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, ESALQ, Dept Entomol & Acarol, Lab Interacoes Insetos, BR-13418900 Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Coll Agr & Veterinarian Sci, Dept Anim Sci, Avian Sci Lab, Sao Paulo, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 11/50877-0
dc.format.extent881-889
dc.identifierhttp://dx.doi.org/10.1007/s00248-014-0463-9
dc.identifier.citationMicrobial Ecology. New York: Springer, v. 68, n. 4, p. 881-889, 2014.
dc.identifier.doi10.1007/s00248-014-0463-9
dc.identifier.issn0095-3628
dc.identifier.urihttp://hdl.handle.net/11449/116253
dc.identifier.wosWOS:000344075300022
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMicrobial Ecology
dc.relation.ispartofjcr3.614
dc.relation.ispartofsjr1,272
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titlePopulation Dynamics and Growth Rates of Endosymbionts During Diaphorina citri (Hemiptera, Liviidae) Ontogenyen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer

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