A cell wall protein-based vaccine candidate induce protective immune response against Sporothrix schenckii infection

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dc.contributor.authorPortuondo, Deivys Leandro [UNESP]
dc.contributor.authorBatista-Duharte, Alexander
dc.contributor.authorFerreira, Lucas Souza [UNESP]
dc.contributor.authorMartínez, Damiana Téllez [UNESP]
dc.contributor.authorPolesi, Marisa Campos [UNESP]
dc.contributor.authorDuarte, Roberta Aparecida [UNESP]
dc.contributor.authorde Paula e Silva, Ana Carolina Alves [UNESP]
dc.contributor.authorMarcos, Caroline Maria [UNESP]
dc.contributor.authorAlmeida, Ana Marisa Fusco de [UNESP]
dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionMedical Science University
dc.date.accessioned2018-12-11T17:06:13Z
dc.date.available2018-12-11T17:06:13Z
dc.date.issued2016-02-01
dc.description.abstractSporotrichosis is a subcutaneous mycosis caused by several closely related thermo-dimorphic fungi of the Sporothrix schenckii species complex, affecting humans and other mammals. In the last few years, new strategies have been proposed for controlling sporotrichosis owning to concerns about its growing incidence in humans, cats, and dogs in Brazil, as well as the toxicity and limited efficacy of conventional antifungal drugs. In this study, we assessed the immunogenicity and protective properties of two aluminum hydroxide (AH)-adsorbed S. schenckii cell wall protein (ssCWP)-based vaccine formulations in a mouse model of systemic S. schenckii infection. Fractioning by SDS-PAGE revealed nine protein bands, two of which were functionally characterized: a 44 kDa peptide hydrolase and a 47 kDa enolase, which was predicted to be an adhesin. Sera from immunized mice recognized the 47 kDa enolase and another unidentified 71 kDa protein, whereas serum from S. schenckii-infected mice recognized both these proteins plus another unidentified 9.4 kDa protein. Furthermore, opsonization with the anti-ssCWP sera led to markedly increased phagocytosis and was able to strongly inhibit the fungus’ adhesion to fibroblasts. Immunization with the higher-dose AH-adjuvanted formulation led to increased ex vivo release of IL-12, IFN-γ, IL-4, and IL-17, whereas only IL-12 and IFN-γ were induced by the higher-dose non-adjuvanted formulation. Lastly, passive transference of the higher-dose AH-adjuvanted formulation's anti-ssCWP serum was able to afford in vivo protection in a subsequent challenge with S. schenckii, becoming a viable vaccine candidate for further testing.en
dc.description.affiliationDepartment of Clinical Analysis Araraquara's School of Pharmaceutical Sciences Universidade Estadual Paulista-UNESP, Júlio Mesquita Filho, Rua Expedicionários do Brasil, Postal Code: 14801-902
dc.description.affiliationImmunotoxicology Laboratory Toxicology and Biomedicine Center (TOXIMED) Medical Science University, Autopista Nacional Km. 1 1/2CP 90400
dc.description.affiliationUnespDepartment of Clinical Analysis Araraquara's School of Pharmaceutical Sciences Universidade Estadual Paulista-UNESP, Júlio Mesquita Filho, Rua Expedicionários do Brasil, Postal Code: 14801-902
dc.format.extent300-309
dc.identifierhttp://dx.doi.org/10.1016/j.imbio.2015.10.005
dc.identifier.citationImmunobiology, v. 221, n. 2, p. 300-309, 2016.
dc.identifier.doi10.1016/j.imbio.2015.10.005
dc.identifier.file2-s2.0-84989770995.pdf
dc.identifier.issn1878-3279
dc.identifier.issn0171-2985
dc.identifier.scopus2-s2.0-84989770995
dc.identifier.urihttp://hdl.handle.net/11449/173548
dc.language.isoeng
dc.relation.ispartofImmunobiology
dc.relation.ispartofsjr1,100
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAdjuvant
dc.subjectAluminum
dc.subjectImmunogenicity
dc.subjectSporothrix schenckii
dc.subjectSporotrichosis
dc.subjectVaccine
dc.titleA cell wall protein-based vaccine candidate induce protective immune response against Sporothrix schenckii infectionen
dc.typeArtigo
unesp.author.lattes3716273524139678[9]
unesp.author.orcid0000-0002-2115-8988[9]

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