In Vitro Susceptibility of Environmental Isolates of Exophiala dermatitidis to Five Antifungal Drugs

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dc.contributor.authorDuarte, Ana Paula Miranda [UNESP]
dc.contributor.authorPagnocca, Fernando Carlos [UNESP]
dc.contributor.authorBaron, Noemi Carla [UNESP]
dc.contributor.authorde Souza Carvalho Melhem, Marcia
dc.contributor.authorPalmeira, Gislene Aparecida
dc.contributor.authorde Franceschi de Angelis, Dejanira [UNESP]
dc.contributor.authorAttili-Angelis, Derlene [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionInstituto Adolfo Lutz (IAL)
dc.date.accessioned2014-05-27T11:29:34Z
dc.date.available2014-05-27T11:29:34Z
dc.date.issued2013-06-01
dc.description.abstractSeveral dematiaceous fungi frequently isolated from nature are involved in cases of superficial lesions to lethal cerebral infections. Antifungal susceptibility data on environmental and clinical isolates are still sparse despite the advances in testing methods. The objective of this study was to examine the activities of 5-flucytosine, amphotericin B, itraconazole, voriconazole and terbinafine against environmental isolates of Exophiala strains by minimum inhibition concentration (MIC) determination. The strains were obtained from hydrocarbon-contaminated soil, ant cuticle and fungal pellets from the infrabuccal pocket of attine gynes. Broth microdilution assay using M38-A2 reference methodology for the five antifungal drugs and DNA sequencing for fungal identification were applied. Terbinafine was the most active drug against the tested strains. It was observed that amphotericin B was less effective, notably against Exophiala spinifera, also studied. High MICs of 5-flucytosine against Exophiala dermatitidis occurred. This finding highlights the relevance of studies on the antifungal resistance of these potential opportunistic species. Our results also contribute to a future improvement of the standard methods to access the drug efficacy currently applied to black fungi. © 2012 Springer Science+Business Media Dordrecht.en
dc.description.affiliationCenter for the Study of Social Insects, IB UNESP- São Paulo State University, Rio Claro, SP
dc.description.affiliationDepartment of Biochemistry and Microbiology, IB UNESP-São Paulo State University, Av. 24A, n. 1515, Bela Vista, Rio Claro, SP, 13506-900
dc.description.affiliationDivision of Microbial Resources, CPQBA University of Campinas, Campinas, SP
dc.description.affiliationAdolfo Lutz Public Health Reference Laboratory, Rio Claro, SP
dc.description.affiliationUnespCenter for the Study of Social Insects, IB UNESP- São Paulo State University, Rio Claro, SP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology, IB UNESP-São Paulo State University, Av. 24A, n. 1515, Bela Vista, Rio Claro, SP, 13506-900
dc.format.extent455-461
dc.identifierhttp://dx.doi.org/10.1007/s11046-012-9597-9
dc.identifier.citationMycopathologia, v. 175, n. 5-6, p. 455-461, 2013.
dc.identifier.doi10.1007/s11046-012-9597-9
dc.identifier.issn0301-486X
dc.identifier.lattes8302605179522059
dc.identifier.scopus2-s2.0-84878548760
dc.identifier.urihttp://hdl.handle.net/11449/75491
dc.identifier.wosWOS:000319768900011
dc.language.isoeng
dc.relation.ispartofMycopathologia
dc.relation.ispartofjcr1.476
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAmphotericin Ben
dc.subjectAzoles-flucytosineen
dc.subjectBlack yeasten
dc.subjectCLSIen
dc.subjectTerbinafineen
dc.subjectChaetothyrialesen
dc.subjectExophialaen
dc.subjectExophiala dermatitidisen
dc.subjectExophiala spiniferaen
dc.subjectFungien
dc.titleIn Vitro Susceptibility of Environmental Isolates of Exophiala dermatitidis to Five Antifungal Drugsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes8302605179522059[2]
unesp.author.orcid0000-0002-5026-1933[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais
Artigos - Bioquímica e Microbiologia - IBRC