Variant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)

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dc.contributor.authorHerkenhoff, Marcos Edgar [UNESP]
dc.contributor.authorBovolenta, Luiz A. [UNESP]
dc.contributor.authorBroedel, Oliver
dc.contributor.authordos Santos, Lucilene D. [UNESP]
dc.contributor.authorde Oliveira, Arthur C. [UNESP]
dc.contributor.authorChuffa, Luiz G.A. [UNESP]
dc.contributor.authorRibeiro, Amanda de O. [UNESP]
dc.contributor.authorLupi, Luiz A. [UNESP]
dc.contributor.authorDias, Marco A.D.
dc.contributor.authorHilsdorf, Alexandre W.S.
dc.contributor.authorFrohme, Marcus
dc.contributor.authorPinhal, Danillo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionTechnical University of Applied Sciences Wildau
dc.contributor.institutionUniversity of Mogi das Cruzes
dc.contributor.institutionFederal University of Lavras
dc.date.accessioned2022-04-29T08:36:39Z
dc.date.available2022-04-29T08:36:39Z
dc.date.issued2021-11-01
dc.description.abstractNile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed.en
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationDivision Molecular Biotechnology and Functional Genomics Technical University of Applied Sciences Wildau
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationUnit of Biotechnology University of Mogi das Cruzes
dc.description.affiliationDepartment of Animal Sciences Federal University of Lavras
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent4303-4312
dc.identifierhttp://dx.doi.org/10.1016/j.ygeno.2021.11.008
dc.identifier.citationGenomics, v. 113, n. 6, p. 4303-4312, 2021.
dc.identifier.doi10.1016/j.ygeno.2021.11.008
dc.identifier.issn1089-8646
dc.identifier.issn0888-7543
dc.identifier.scopus2-s2.0-85119451126
dc.identifier.urihttp://hdl.handle.net/11449/229918
dc.language.isoeng
dc.relation.ispartofGenomics
dc.sourceScopus
dc.subjectESI-Q-TOF
dc.subjectGrowth
dc.subjectmiRNA
dc.subjectNile tilapia
dc.subjectProteome
dc.subjectRNA-Seq
dc.titleVariant expression signatures of microRNAs and protein related to growth in a crossbreed between two strains of Nile tilapia (Oreochromis niloticus)en
dc.typeArtigo

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Artigos - Física e Biofísica - IBB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos