Parthenogenetic development of domestic cat oocytes treated with ionomycin, cycloheximide, roscovitine and strontium

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dc.contributor.authorSilva Rascado, T. da [UNESP]
dc.contributor.authorRigatto Martins, L. [UNESP]
dc.contributor.authorWatanabe Minto, B. [UNESP]
dc.contributor.authorSa Lorena, S. E. R. de [UNESP]
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:28:33Z
dc.date.accessioned2014-05-20T13:42:01Z
dc.date.available2013-09-30T18:28:33Z
dc.date.available2014-05-20T13:42:01Z
dc.date.issued2010-09-01
dc.description.abstractThe objective was to evaluate the parthenogenetic activation of domestic cat oocytes. Cumulus-oocyte complexes matured for 36 h were subjected to three protocols of parthenogenetic activation: Group 1 - ionomycin + cycloheximide; Group 2 - ionomycin + roscovitine; and Group 3 - ionomycin + strontium. As a control, a fourth group of oocytes were cultured in the absence of any activation agent. In all groups, embryos were cultured in SOFaa for 72 h after activation and evaluated for activation rate, cleavage, and embryonic development using Hoechst 33342. There were no significant differences among the three treated groups for rates of activated oocytes (70.1 +/- 4.3, 75.5 +/- 4.7, and 61.9 +/- 7.2%, for Treatments 1, 2, and 3 respectively; mean +/- SEM), or cleavage (48.1 +/- 5.9, 47.4 +/- 3.8, and 33.3 +/- 6.8%). However, activation and cleavage rates were higher (P < 0.05) than those in the control group (35.5 +/- 6.4 and 11.8 +/- 4.0%). There were no significant differences among treatment groups for proportion of embryos with 2-10 cells, 10-16 cells, and morulas. In the Control group, the embryo production rate was lower (P < 0.05), although the activation rate was high. The authors concluded that all three treatments effectively induced parthenogenetic activation of domestic cat oocytes. However, to optimize the use of strontium and roscovitine, a dose response and the effect of the presence of Ca(++) in the medium requires further study. (C) 2010 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista FMVZ, UNESP, Fac Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista FMVZ, UNESP, Fac Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista FMVZ, UNESP, Fac Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista FMVZ, UNESP, Fac Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent596-601
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2010.03.010
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 74, n. 4, p. 596-601, 2010.
dc.identifier.doi10.1016/j.theriogenology.2010.03.010
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/14590
dc.identifier.wosWOS:000281261600014
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectStrontiumen
dc.subjectRoscovitineen
dc.subjectCycloheximideen
dc.subjectArtificial activationen
dc.subjectFeline oocytesen
dc.titleParthenogenetic development of domestic cat oocytes treated with ionomycin, cycloheximide, roscovitine and strontiumen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0131201084614247[3]
unesp.author.orcid0000-0002-9212-0768[2]
unesp.author.orcid0000-0002-8808-4127[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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