Genotoxicity and Fetal Abnormality in Streptozotocin-Induced Diabetic Rats Exposed to Cigarette Smoke Prior to and during Pregnancy

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dc.contributor.authorDamasceno, Débora Cristina [UNESP]
dc.contributor.authorVolpato, Gustavo Tadeu [UNESP]
dc.contributor.authorSinzato, Y. K. [UNESP]
dc.contributor.authorLima, P. H. O. [UNESP]
dc.contributor.authorSouza, M. S. S. [UNESP]
dc.contributor.authorIessi, I. L. [UNESP]
dc.contributor.authorKiss, A. C. I. [UNESP]
dc.contributor.authorTakaku, M. [UNESP]
dc.contributor.authorRudge, Marilza Vieira Cunha [UNESP]
dc.contributor.authorCalderon, Iracema de Mattos Paranhos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Mato Grosso (UFMT)
dc.contributor.institutionUniversidade de Marília (UNIMAR)
dc.date.accessioned2014-05-20T13:35:27Z
dc.date.available2014-05-20T13:35:27Z
dc.date.issued2011-10-01
dc.description.abstractBackground: Maternal hyperglycemia during early pregnancy is associated with increased risk of abnormalities in the off spring. Malformation rates among the off spring of diabetic mothers are 2-5-fold higher than that of the normal population, and congenital malformations are the major cause of mortality and morbidity in the off spring of diabetic mothers. Metabolic changes, such as hyperglycemia and the metabolites obtained from cigarettes both increase the production of reactive oxygen species (ROS) in the embryo or fetus, causing DNA damage.Objective: To evaluate the maternal and fetal genotoxicity, and to assess the incidence of fetal anomaly in diabetic female rats exposed to cigarette smoke at different stages of pregnancy in rats.Material and Method: Diabetes was induced by streptozotocin administration and cigarette smoke exposure was produced by a mechanical smoking device that generated mainstream smoke that was delivered into a chamber. Female Wistar rats were randomly assigned to: non-diabetic (ND) and diabetic (D) groups exposed to filtered air; a diabetic group exposed to cigarette smoke prior to and during pregnancy (DS) and a diabetic group only exposed to cigarette smoke prior to pregnancy (DSPP). on pregnancy day 21, blood samples were obtained for DNA damage analysis and fetuses were collected for congenital anomaly assessment. Statistical significance was set at p<0.05 for all analysis.Results and Conclusion: Exposure of diabetic rats to tobacco smoke prior to pregnancy increased fetal DNA damage, but failed to induce teratogenicity. Thus, these results reinforce the importance for women to avoid exposure to cigarette smoke long before they become pregnant.en
dc.description.affiliationUniv Estadual Paulista UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, SP, Brazil
dc.description.affiliationMato Grosso Fed Univ UFMT, Univ Ctr Araguaia, Inst Biol & Hlth Sci, Cuiaba, Mato Grosso Sta, Brazil
dc.description.affiliationUniv Marilia Unimar, Pharmacol Course, Marilia, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, São Paulo, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 04/01077-8
dc.format.extent549-553
dc.identifierhttp://dx.doi.org/10.1055/s-0031-1277193
dc.identifier.citationExperimental and Clinical Endocrinology & Diabetes. Stuttgart: Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh, v. 119, n. 9, p. 549-553, 2011.
dc.identifier.doi10.1055/s-0031-1277193
dc.identifier.issn0947-7349
dc.identifier.lattes6758680388835078
dc.identifier.lattes0679387622604743
dc.identifier.orcid0000-0002-9227-832X
dc.identifier.urihttp://hdl.handle.net/11449/12197
dc.identifier.wosWOS:000296207400007
dc.language.isoeng
dc.publisherJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
dc.relation.ispartofExperimental and Clinical Endocrinology & Diabetes
dc.relation.ispartofjcr1.623
dc.relation.ispartofsjr0,695
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdiabetesen
dc.subjectcigarette smokeen
dc.subjectpregnancyen
dc.subjectgenotoxicityen
dc.subjectfetal anomalyen
dc.subjectraten
dc.titleGenotoxicity and Fetal Abnormality in Streptozotocin-Induced Diabetic Rats Exposed to Cigarette Smoke Prior to and during Pregnancyen
dc.typeArtigo
dcterms.licensehttp://www.thieme.de/fz/_pdf/thieme_ir_policy_english.pdf
dcterms.rightsHolderJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
unesp.author.lattes6758680388835078
unesp.author.lattes0679387622604743
unesp.author.lattes1490222400024738[7]
unesp.author.orcid0000-0002-9227-832X[9]
unesp.author.orcid0000-0002-4753-3264[2]
unesp.author.orcid0000-0003-4761-4336[10]
unesp.author.orcid0000-0002-7003-9643[1]
unesp.author.orcid0000-0002-8558-0141[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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