Analysis of DNA damage induced by aflatoxin B1 in Dunkin-Hartley guinea pigs

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dc.contributor.authorMiranda, D. D C
dc.contributor.authorArçari, D. P.
dc.contributor.authorLadeira, M. S P [UNESP]
dc.contributor.authorCalori-Domingues, M. A.
dc.contributor.authorRomero, A. C.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.authorGloria, E. M.
dc.contributor.authorPedrazzoli, J.
dc.contributor.authorRibeiro, M. L.
dc.contributor.institutionUniversidade São Francisco
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:22:27Z
dc.date.available2014-05-27T11:22:27Z
dc.date.issued2007-05-01
dc.description.abstractAflatoxin B1 (AFB1) is among the most potent naturally occurring carcinogens and classified as a group I carcinogen. Since the ingestion of aflatoxin-contaminated food is associated with several liver diseases, the aim of the present study was to evaluate the effect of 2, 20, and 200 ppb of AFB1 on DNA damage in peripheral blood lymphocytes and liver cells in Dunkin-Hartley guinea pigs. The animals were divided into four groups according to the given diet. After the treatment the lymphocytes and liver cells were isolated and DNA damage determined by Comet assay. The levels of DNA damage in lymphocytes were higher animals treated with 200 ppb of AFB1-enriched diet (P = 0.02). In the liver cells there were a relationship between the levels of DNA damage and the consumption of AFB1 in all studied groups. These results suggest that Comet assay performed on lymphocytes is a valuable genotoxic marker for high levels of exposure to AFB1 in guinea pig. Additionally our results indicate that the exposure to this toxin increases significantly and increases the level of DNA damage in liver cells, which is a key step on liver cancer development. We also suggest that the Comet assay is an useful tool for monitoring the genotoxicity of AFB1 in liver. © 2007 Springer Science+Business Media B.V.en
dc.description.affiliationUnidade Integrada de Farmacologia e Gastroenterologia Universidade São Francisco, Av. São Francisco de Assis, Braganca Paulista, SP 12916-900
dc.description.affiliationDepto. de Patologia Faculdade de Medicina UNESP, Botucatu, SP
dc.description.affiliationDepto. Agroindústria, Alimentos e Nutrição ESALQ USP, Piracicaba, SP
dc.description.affiliationUnespDepto. de Patologia Faculdade de Medicina UNESP, Botucatu, SP
dc.format.extent275-280
dc.identifierhttp://dx.doi.org/10.1007/s11046-007-9014-y
dc.identifier.citationMycopathologia, v. 163, n. 5, p. 275-280, 2007.
dc.identifier.doi10.1007/s11046-007-9014-y
dc.identifier.issn0301-486X
dc.identifier.lattes5051118752980903
dc.identifier.scopus2-s2.0-34248196544
dc.identifier.urihttp://hdl.handle.net/11449/69635
dc.language.isoeng
dc.relation.ispartofMycopathologia
dc.relation.ispartofjcr1.476
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAflatoxin B1
dc.subjectComet Assay
dc.subjectOxidative DNA damage
dc.subjectaflatoxin B1
dc.subjectcarcinogen
dc.subjectmutagenic agent
dc.subjectanimal
dc.subjectcomet assay
dc.subjectDNA damage
dc.subjectdrug effect
dc.subjectfood contamination
dc.subjectguinea pig
dc.subjectliver cell
dc.subjectlymphocyte
dc.subjectmale
dc.subjectmetabolism
dc.subjectAnimals
dc.subjectCarcinogens
dc.subjectDNA Damage
dc.subjectFood Contamination
dc.subjectGuinea Pigs
dc.subjectHepatocytes
dc.subjectLymphocytes
dc.subjectMale
dc.subjectMutagens
dc.subjectAnimalia
dc.subjectCavia
dc.titleAnalysis of DNA damage induced by aflatoxin B1 in Dunkin-Hartley guinea pigsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes5051118752980903
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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