Controle epigenético de genes imprinted em placentas de gestações produzidas por transferência embrionária, fecundação in vitro e transferência nuclear em bovinos

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dc.contributor.advisorLopes, Flavia Lombardi [UNESP]
dc.contributor.authorPenteado, João Carlos Torrente [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2016-09-27T13:39:59Z
dc.date.available2016-09-27T13:39:59Z
dc.date.issued2015-12-09
dc.description.abstractThe process of embryonic and placental development is under high epigenetic control, with different epigenetic processes regulating the differentiation of pluripotent cells into specialized cells. An abnormal placental development is observed frequently in pregnancies produced by nuclear transfer, and is associated with incomplete epigenetic reprogramming of the donor cell and formed embryo. The objective of this study was to evaluate the expression of the imprinted genes TSSC4 e PHLDA2 in cotiledonary and intercotiledonary tissues, after 60 days of pregnancy, in placentas produced by Embryo Transfer (ET) In Vitro Fertilization (IVF) and Nuclear Transfer (NT) in cattle, and also to investigate the epigenetic control of this gene in the bovine placenta evaluating the epigenetic profile of cotiledonary tissues of the experimental groups (ET, IVF and NT). The TSSC4 gene expression was reduced by 30% in the cotyledons in the NT group, however PHLDA2 expression of the gene was elevated by 11-fold in NT compared to ET. ChIP analysis for histone H3 at the proximal promoter region of TSSC4 and PHLDA2 genes showed an increase to the permissive mark H3K4me2 and a reduction for the inhibitory mark H3K9me2 in the cotyledons of cloned placentae, in relation to placentae produced by ET, for both genes. Interestingly, the inhibitory mark H3K9me2 for TSSC4 gene was also significantly reduced in placentae produced by IVF, compared to ET control group. These results show that expression of the imprinted genes TSSC4 and PHLDA2 is affected in placenta of pregnancies produced by nuclear transfer. Further, changes in expression of gene PHLDA2 and histone modifications in TSSC4 gene cotyledons produced by IVF indicate that the in vitro culture can contribute to the observed changes in NT group.en
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent63 f.
dc.identifier.aleph000870620
dc.identifier.capes33004021075P8
dc.identifier.citationPENTEADO, João Carlos Torrente. Controle epigenético de genes imprinted em placentas de gestações produzidas por transferência embrionária, fecundação in vitro e transferência nuclear em bovinos. 2015. 63 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária, 2015.
dc.identifier.filehttp://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/08-09-2016/000870620.pdf
dc.identifier.urihttp://hdl.handle.net/11449/144049
dc.language.isopor
dc.publisherUniversidade Estadual Paulista (Unesp)
dc.rights.accessRightsAcesso aberto
dc.sourceAleph
dc.subjectBovinopt
dc.subjectBiotecnologia animalpt
dc.subjectPlacentapt
dc.subjectTransferência de embriõespt
dc.subjectInseminação artificialpt
dc.subjectTécnicas de maturação in vitro de oócitospt
dc.subjectPHLDA2pt
dc.titleControle epigenético de genes imprinted em placentas de gestações produzidas por transferência embrionária, fecundação in vitro e transferência nuclear em bovinospt
dc.typeDissertação de mestrado
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt
unesp.graduateProgramCiência Animal - FMVApt
unesp.knowledgeAreaMedicina Veterinária Preventiva e Produção Animalpt
unesp.researchAreaEpidemiologia, etiopatogenia, diagnóstico e controle das enfermidades dos animaispt

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Dissertações - Ciência Animal - FMVA