Avaliação experimental dos efeitos do anestésico local ropivacaína em nervos ciáticos

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dc.contributor.authorZittlau, Mara Lúcia Costamilan
dc.contributor.authorTaboga, Sebastião Roberto [UNESP]
dc.contributor.institutionLetras Cie. Exatas da Univ. E.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:24Z
dc.date.available2014-05-27T11:20:24Z
dc.date.issued2002-01-01
dc.description.abstractThis study was undertaken to investigate the effects of ropivacaine after intrafascicular injection into the sciatic nerves of albino rabbits. Twenty adult albine rabbits were used, following sedation with intramuscular ketamine (50 mg/kg) for nerve exposure by lateral incision. We considered three experimental groups: Group I:sciatic nerve control; Group II: intrafascicular injection with 0.2 mL of physiologic saline solution in the left nerves and intrafascicular injection with 0.2 mL of local anesthetic ropivacaine into the rigth nerves. The specimens were colected at 48 h after drugs administration; Group III. intrafascicular injection with 0.2 mL of physiologic saline solution in the left nerves and intrafascicular injection with 0.2 mL of local anesthetic ropivacaine in the rigth nerves. The specimens were colected at 7 days after drugs administration. The sciatic nerves were removed from these animals and fixed in Karnowisky solution for 24 hours. After partial dehydration up to 95% ethanol, they were embedded in historesin (Leica). The tissue was then sectioned at 1-2μm. Sections were stained with haematoxylin-eosin (HE); toluidine blue (TB) or picrosirius-haematoxylin (PSH). Comparing with control group the histological evidence of inflammatory reaction (migration of macrophagic cells and eosinophils-appeared soon after injection, with intense proliferation of perineurial cells. The results show that after 7 days of intrafascicular injection there was a severe fibrosis and an increase on perineurial vascularization. In group 2 the inflammatory reaction was noted near the local of the injection. Furthermore in this experiment we observed an increase on the number of epineurial lipoblasts and adipocytes. This study demonstrated that the toxic effects of ropivacaine are transient. In many cases there was an initial fascicular recover and axonal regeneration after 7 days of the injection.en
dc.description.affiliationDepartamento de Biologia Instituto de Biociências Letras Cie. Exatas da Univ. E., Sao Jose do Rio Preto - SP
dc.description.affiliationDepartamento de Biologia/IBILCE UNESP, R. C. C., 2265 - Jardim Nazareth, Sao Jose do Rio Preto - SP
dc.description.affiliationUnespDepartamento de Biologia/IBILCE UNESP, R. C. C., 2265 - Jardim Nazareth, Sao Jose do Rio Preto - SP
dc.format.extent89-94
dc.identifierhttp://www.moreirajr.com.br/revistas.asp?id_materia=1802&fase=imprime
dc.identifier.citationRevista Brasileira de Medicina, v. 59, n. 1-2, p. 89-94, 2002.
dc.identifier.issn0034-7264
dc.identifier.lattes1445259468526188
dc.identifier.orcid0000-0002-0970-4288
dc.identifier.scopus2-s2.0-0344986480
dc.identifier.urihttp://hdl.handle.net/11449/66789
dc.language.isopor
dc.relation.ispartofRevista Brasileira de Medicina
dc.relation.ispartofsjr0,101
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectHistology
dc.subjectNeurotoxicity
dc.subjectRopivacaine
dc.subjectSciatic nerve
dc.subjectalcohol
dc.subjecteosin
dc.subjectfixative
dc.subjecthematoxylin
dc.subjectketamine
dc.subjectlocal anesthetic agent
dc.subjectresin
dc.subjectropivacaine
dc.subjectsodium chloride
dc.subjecttolonium chloride
dc.subjectxylazine
dc.subjectadipocyte
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectcell proliferation
dc.subjectcontrolled study
dc.subjectdrug administration route
dc.subjectdrug effect
dc.subjecteosinophil
dc.subjectfibrosis
dc.subjecthistology
dc.subjectinflammation
dc.subjectmacrophage migration
dc.subjectnerve regeneration
dc.subjectneurotoxicity
dc.subjectnonhuman
dc.subjectperineurium
dc.subjectrabbit
dc.subjectsciatic nerve
dc.subjectsedation
dc.subjectvascularization
dc.titleAvaliação experimental dos efeitos do anestésico local ropivacaína em nervos ciáticospt
dc.title.alternativeExperimental evaluation of the local anesthetic ropivacaine effects on sciatic nervesen
dc.typeArtigo
unesp.author.lattes1445259468526188
unesp.author.orcid0000-0002-0970-4288[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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