Effect of berberine nanoemulsion Photodynamic therapy on cervical carcinoma cell line

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dc.contributor.authorFloriano, Barbara Freitas [UNESP]
dc.contributor.authorCarvalho, Tamara [UNESP]
dc.contributor.authorLopes, Tairine Zara [UNESP]
dc.contributor.authorTakahashi, Luandra Aparecida Unten
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.authorTedesco, Antonio Claudio
dc.contributor.authorCalmon, Marília Freitas [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2021-06-25T10:49:18Z
dc.date.available2021-06-25T10:49:18Z
dc.date.issued2021-03-01
dc.description.abstractCervical carcinoma is the most common gynecological cancer among young and adult women. There has been increasing interest in natural sources for cervical carcinoma treatment, especially for active compounds from plant extracts as antineoplastic agents. Berberine is an example of one these promising natural products. It is a natural isoquinoline alkaloid and comes from plants, such as Berbis, Coptis, and Hydrastis. It is widely used in Chinese medicine and has demonstrated activity against various cancer cell lines. This work aims to analyze the efficiency of berberine-containing nanoemulsions as photosensitizing agents in photodynamic therapy and their interaction with cervical carcinoma cells and immortalized human keratinocyte cell line. Among all groups tested, berberine nanoemulsions combined with photodynamic therapy induced the most statistically significant phototoxicity in the evaluated cell lines. Fluorescence microscopy demonstrated that the compound was present for up to 48 h when berberine nanoemulsions were used. The reactive oxygen species assay showed an increase in reactive oxygen species in the two studied cell lines after treatment of berberine-containing nanoemulsion combined with photodynamic therapy. The autophagy trial showed significant increases in cell death when berberine-containing nanoemulsion treatment was combined with photodynamic therapy when compared to trichostatin A treatment as a positive control. However, caspase-3 activity did not significantly increase in cervical carcinoma cells and immortalized human keratinocyte cell line. The results suggest that nanoemulsions with berberine have potential for use as photosensitizing agents in photodynamic therapy to treat cervical carcinoma.en
dc.description.affiliationUNESP São Paulo State University IBILCE - Institute of Biosciences Humanities and Exact Sciences Department of Biology, Rua Cristóvão Colombo, 2265 - Bairro Jardim Nazareth
dc.description.affiliationDepartment of Chemistry Center for Nanotechnology and Tissue Engineering Faculty of Philosophy Sciences and Languages of Ribeirão Preto University of São Paulo USP, Ribeirão Preto
dc.description.affiliationUnespUNESP São Paulo State University IBILCE - Institute of Biosciences Humanities and Exact Sciences Department of Biology, Rua Cristóvão Colombo, 2265 - Bairro Jardim Nazareth
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/16660-5
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2020.102174
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 33.
dc.identifier.doi10.1016/j.pdpdt.2020.102174
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85099218452
dc.identifier.urihttp://hdl.handle.net/11449/207119
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectBerberine
dc.subjectCervical cancer
dc.subjectNanoemulsions
dc.subjectPhotodynamic therapy
dc.titleEffect of berberine nanoemulsion Photodynamic therapy on cervical carcinoma cell lineen
dc.typeArtigo
unesp.author.orcid0000-0002-0114-8379[2]
unesp.author.orcid0000-0001-9614-5666[4]
unesp.author.orcid0000-0001-5203-0103[7]

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