Extracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production, and Application

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dc.contributor.authorAracri, Fernanda Mansano [UNESP]
dc.contributor.authorFarias Cavalcanti, Rayza Morganna [UNESP]
dc.contributor.authorSouza Guimaraes, Luis Henrique
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2020-12-10T17:01:23Z
dc.date.available2020-12-10T17:01:23Z
dc.date.issued2019-11-01
dc.description.abstractAspergillus ochraceus biofilm, developed on an inert support, can produce tannase in Khanna medium containing 1.5% (w/v) tannic acid as the carbon source, at an initial pH of 5.0, for 72 h at 28 degrees C. Addition of 0.1% (w/v) yeast extract increased enzyme production. The enzyme in the crude filtrate exhibited the highest activity at 30 degrees C and pH 6.0. At 50 degrees C, the half-life was 60 min and 260 min at pH 6.0. In general, addition of detergents and surfactants did not affect tannase activity significantly. Tannase has potential applications in various biotechnological processes such as the production of propyl gallate and in the treatment of tannin-rich effluents. The content of tannins and total phenolic compounds in effluents from leather treatment was reduced by 56-83% and 47-64%, respectively, after 2 h of enzyme treatment. The content of tannins and total phenolic compounds in the sorghum flour treated for 120 h with tannase were reduced by 61% and 17%, respectively. Interestingly, the same A. ochraceus biofilm was able to produce tannase for three sequential fermentative process. In conclusion, fungal biofilm is an interesting alternative to produce high levels of tannase with biotechnological potential to be applied in different industrial sectors.en
dc.description.affiliationUNESP, Inst Quim Araraquara, Ave Prof Mario Degni S-N, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ave Bandeirantes 3900, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Quim Araraquara, Ave Prof Mario Degni S-N, BR-14800900 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2016/11311-5
dc.format.extent1749-1759
dc.identifierhttp://dx.doi.org/10.4014/jmb.1903.03060
dc.identifier.citationJournal Of Microbiology And Biotechnology. Seoul: Korean Soc Microbiology & Biotechnology, v. 29, n. 11, p. 1749-1759, 2019.
dc.identifier.doi10.4014/jmb.1903.03060
dc.identifier.issn1017-7825
dc.identifier.urihttp://hdl.handle.net/11449/195006
dc.identifier.wosWOS:000499146400008
dc.language.isoeng
dc.publisherKorean Soc Microbiology & Biotechnology
dc.relation.ispartofJournal Of Microbiology And Biotechnology
dc.sourceWeb of Science
dc.subjectBiofilm fermentation
dc.subjectfungal biofilm
dc.subjectleather effluent
dc.subjecttannase
dc.subjecttannin acyl hydrolase
dc.titleExtracellular Tannase from Aspergillus ochraceus: Influence of the Culture Conditions on Biofilm Formation, Enzyme Production, and Applicationen
dc.typeArtigo
dcterms.rightsHolderKorean Soc Microbiology & Biotechnology

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