Low-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracil

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dc.contributor.authorTheodoro, Leticia Helena [UNESP]
dc.contributor.authorLongo, Mariéllen [UNESP]
dc.contributor.authorNovaes, Vivian Cristina Noronha [UNESP]
dc.contributor.authorMiessi, Daniela Maria Janjacomo [UNESP]
dc.contributor.authorFerro-Alves, Marcio Luiz [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorde Almeida, Juliano Milanezi [UNESP]
dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:32:26Z
dc.date.available2018-12-11T17:32:26Z
dc.date.issued2017-10-01
dc.description.abstractPurpose: The aim of this study was to evaluate the effects of low-level laser therapy (LLLT) and antimicrobial photodynamic therapy (aPDT) as adjuvant to mechanical treatment of experimental periodontitis (EP) in adult rats submitted to 5-fluorouracil (5-FU) chemotherapy. Methods: EP was induced through ligature around the left mandibular first molar for 7 days. The ligature was removed and the animals separated into groups: EP, no treatment; 5FU, systemic administration of 5-FU (80 and 40 mg/kg); 5FU/scaling and root planing (SRP), systemic application of 5-FU and SRP; 5FU/SRP/LLLT, systemic application of 5-FU, SRP, and LLLT (660 nm, 0.035 W; 29.4 J/cm2); and 5FU/SRP/aPDT, systemic application of 5-FU, SRP, and aPDT (methylene blue irrigation and LLLT). The animals were euthanized 7, 15, and 30 days after treatments. Histological sections from mandibles were processed for histomorphometric and immunohistochemical analysis (TRAP, RANKL, OPG, TNF-α, IL-6, IL-10). The alveolar bone loss (BL) area in the furcation region of the mandibular first molar was analyzed histometrically. Results: There was less bone loss in 5FU/SRP/aPDT compared with 5FU at 7 days (p < 0.05). The immunohistochemical analysis showed no significant difference for TRAP and osteoprotegerin, but lower RANKL immunolabeling was observed in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups compared with the 5FU group at 15 days. There was lower TNF-α and IL-6 immunolabeling in the 5FU/SRP/LLLT and 5FU/SRP/aPDT groups and higher IL-10 immunolabeling in 5FU/SRP/aPDT at 30 days. Conclusion: LLLT and aPDT adjuvant to SRP minimized the effects of 5-FU on periodontal disease. Furthermore, aPDT promoted greater benefits in bone loss control and inflammatory response.en
dc.description.affiliationDivision of Periodontology Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationDivision of Histology and Embryology Department of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDivision of Periodontology Department of Surgery and Integrated Clinic School of Dentistry São Paulo State University (Unesp)
dc.description.affiliationUnespDivision of Histology and Embryology Department of Basic Science School of Dentistry São Paulo State University (Unesp)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 2309447-2010-0
dc.format.extent3261-3271
dc.identifierhttp://dx.doi.org/10.1007/s00520-017-3738-0
dc.identifier.citationSupportive Care in Cancer, v. 25, n. 10, p. 3261-3271, 2017.
dc.identifier.doi10.1007/s00520-017-3738-0
dc.identifier.file2-s2.0-85019078776.pdf
dc.identifier.issn1433-7339
dc.identifier.issn0941-4355
dc.identifier.lattes4408095517346846
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.scopus2-s2.0-85019078776
dc.identifier.urihttp://hdl.handle.net/11449/178858
dc.language.isoeng
dc.relation.ispartofSupportive Care in Cancer
dc.relation.ispartofsjr1,297
dc.relation.ispartofsjr1,297
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectChemotherapy
dc.subjectLasers
dc.subjectPeriodontitis
dc.subjectPhotochemotherapy
dc.titleLow-level laser and antimicrobial photodynamic therapy on experimental periodontitis in rats submitted to chemotherapy by 5-fluorouracilen
dc.typeArtigo
unesp.author.lattes4408095517346846[6]
unesp.author.orcid0000-0003-4859-0583[6]

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Artigos - Cirurgia e Clínica Integrada - FOA
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