Mg–Al and Zn–Al Layered Double Hydroxides Promote Dynamic Expression of Marker Genes in Osteogenic Differentiation by Modulating Mitogen-Activated Protein Kinases

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dc.contributor.authorKang, Ha Ram [UNESP]
dc.contributor.authorda Costa Fernandes, Célio Junior [UNESP]
dc.contributor.authorda Silva, Rodrigo Augusto [UNESP]
dc.contributor.authorConstantino, Vera Regina Leopoldo
dc.contributor.authorKoh, Ivan Hong Jun
dc.contributor.authorZambuzzi, Willian F. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-12-11T17:17:05Z
dc.date.available2018-12-11T17:17:05Z
dc.date.issued2018-02-21
dc.description.abstractThe effect of LDH samples comprised of chloride anions intercalated between positive layers of magnesium/aluminum (Mg-Al LDH) or zinc/aluminum (Zn-Al LDH) chemical composition on pre-osteoblast performance is investigated. Non-cytotoxic concentrations of both LDHs modulated pre-osteoblast adhesion by triggering cytoskeleton rearrangement dependent on recruiting of Cofilin, which is modulated by the inhibition of the Protein Phosphatase 2A (PP2A), culminating in osteoblast differentiation with a significant increase of osteogenic marker genes. The alkaline phosphatase (ALP) and bone sialoprotein (BSP) are significantly up-modulated by both LDHs; however, Mg-Al LDH nanomaterial promoted even more significance than both experimental controls, while the phosphorylations of mitogen-activated protein kinase (MAPKs)- extracellular signal–regulated kinases (ERK) and c-Jun N-terminal kinase (JNK) significantly increased. MAPK signaling is necessary to activate Runt-related transcription factor 2 (RUNX2) gene. Concomitantly, it is also investigated whether challenged osteoblasts are able to modulate osteoclastogenesis by investigating both osteoprotegerin (OPG) and Receptor activator of nuclear factor kappa-ligand (RANKL) in this model; a dynamic reprogramming of both these genes is found, suggesting LDHs in modulating osteoclastogenesis. These results suggest that LDHs interfere in bone remodeling, and they can be considered as nanomaterials in graft-based bone healing or drug-delivery materials for bone disorders.en
dc.description.affiliationLaboratorio de Bioensaios e Dinâmica Celular Departamento de Química e Bioquímica Instituto de Biociências Universidade Estadual Paulista–UNESP, campus Botucatu
dc.description.affiliationDepartamento de Química Fundamental Instituto de Química Universidade de São Paulo–USP, Av. Prof. Lineu Prestes 748
dc.description.affiliationDepartamento de Cirurgia Universidade Federal de São Paulo–UNIFESP, Rua Botucatu 740
dc.description.affiliationUnespLaboratorio de Bioensaios e Dinâmica Celular Departamento de Química e Bioquímica Instituto de Biociências Universidade Estadual Paulista–UNESP, campus Botucatu
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/50318-1
dc.description.sponsorshipIdFAPESP: 2014/22689-3
dc.identifierhttp://dx.doi.org/10.1002/adhm.201700693
dc.identifier.citationAdvanced Healthcare Materials, v. 7, n. 4, 2018.
dc.identifier.doi10.1002/adhm.201700693
dc.identifier.issn2192-2659
dc.identifier.issn2192-2640
dc.identifier.scopus2-s2.0-85039039972
dc.identifier.urihttp://hdl.handle.net/11449/175686
dc.language.isoeng
dc.relation.ispartofAdvanced Healthcare Materials
dc.relation.ispartofsjr1,989
dc.relation.ispartofsjr1,989
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectdifferentiation
dc.subjectdouble metal hydroxides
dc.subjecthydrotalcite-like materials
dc.subjectlayered double hydroxides
dc.subjectnanoparticles
dc.subjectosteoblasts
dc.titleMg–Al and Zn–Al Layered Double Hydroxides Promote Dynamic Expression of Marker Genes in Osteogenic Differentiation by Modulating Mitogen-Activated Protein Kinasesen
dc.typeArtigo
unesp.author.orcid0000-0002-4149-5965[6]

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