Avaliação da composição salivar e estado redox das glândulas salivares de ratos Wistar portadores de periodontite apical induzida
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2024-02-27
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Universidade Estadual Paulista (Unesp)
Resumo
O objetivo do estudo foi avaliar os efeitos da periodontite apical experimental nos parâmetros inflamatórios, funcionais, bioquímicos e redox das glândulas parótida e submandibular em ratos. Vinte ratos Wistar machos, com 12 semanas de idade, foram divididos aleatoriamente em dois grupos (n = 10): grupo controle, sem lesões induzidas e grupo periodontite apical, com 4 dentes com lesões induzidas. Após 28 dias, a saliva estimulada por pilocarpina foi coletada para análise da taxa de fluxo salivar e composição bioquímica (proteína total, amilase e eletrólitos). As glândulas parótida e submandibular foram coletadas para quantificação do fator de necrose tumoral-alfa (TNF-α), capacidade oxidativa total, peroxidação lipídica, expressa como substância reativa ao ácido tiobarbitúrico, proteína carbonila, capacidade antioxidante total, glutationa, superóxido dismutase, catalase e glutationa peroxidase. As análises estatísticas foram realizadas usando o teste t de Student pareado (esquerda versus direita) e o teste t de Student não pareado (controle versus periodontite apical), p < 0,05. As concentrações de TNF-α foram mais elevadas em ambas as glândulas salivares adjacentes às lesões periapicais em animais com periodontite apical (p < 0,05), e também em comparação com o grupo controle (p < 0,01). Embora a taxa de fluxo salivar, o pH e a capacidade tampão fossem semelhantes entre os grupos, o grupo com periodontite apical aumentou a amilase salivar (p < 0,01), cloreto (p < 0,05), potássio (p < 0,01), cálcio (p < 0,01) e fosfato (p < 0,05). A capacidade oxidante total aumentou na glândula parótida adjacente às lesões periapicais (p < 0,001) no mesmo rato e em comparação com o grupo controle (p < 0,05). Em contraste, a peroxidação lipídica e proteica não diferiram entre os grupos em nenhuma das glândulas salivares. Pelo contrário, a capacidade antioxidante total das glândulas parótidas em ambos os lados no grupo com periodontite apical foi menor do que no grupo controle (p < 0,05). Além disso, a atividade da glutationa peroxidase aumentou na glândula submandibular adjacente ao grupo com periodontite apical em comparação com o grupo controle (p < 0,05), enquanto as atividades de glutationa, superóxido dismutase e catalase não foram alteradas pela periodontite apical. Conclui-se que a periodontite apical experimental altera a composição bioquímica salivar, além de aumentar os marcadores inflamatórios e induzir distúrbios locais no estado redox nas glândulas parótidas e submandibulares de ratos machos.
The objective of the study was to evaluate the effects of experimental apical periodontitis on the inflammatory, functional, biochemical, and redox parameters of the parotid and submandibular glands in rats. Twenty 12-week-old male Wistar rats were randomly divided into two groups (n = 10): a control group, without induced lesions and apical periodontitis group, with 4 teeth with induced lesions. After 28 days, pilocarpine stimulated saliva was collected for salivary flow rate and biochemistry composition analysis (total protein, amylase, and electrolytes). The parotid and submandibular glands were sampled for quantification of the tumor necrosis factor-alpha (TNF-α), total oxidative capacity, lipid peroxidation, expressed as thiobarbituric acid-reactive substance, carbonyl protein, total antioxidant capacity, glutathione, superoxide dismutase, catalase, and glutathione peroxidase. Statistical analyses were conducted using the paired Student's t-test (left versus right) and unpaired Student's t-test (control versus apical periodontitis), p < 0.05. TNF-α concentrations were higher in both salivary glands adjacent to the periapical lesions in animals with apical periodontitis (p < 0.05), and also compared to the control group (p < 0.01). Although the salivary flow rate, pH, and buffer capacity were similar between groups, the apical periodontitis group increased the salivary amylase (p < 0.01), chloride (p < 0.05), potassium (p < 0.01), calcium (p < 0.01), and phosphate (p < 0.05). The total oxidant capacity increased in the parotid gland adjacent to the periapical lesions (p < 0.001) in the same rat and compared to the control group (p < 0.05). In contrast, lipid and protein peroxidation did not differ between groups in either salivary gland. Conversely, the total antioxidant capacity of the parotid glands on both sides in the apical periodontitis group was lower than the control group (p < 0.05). Furthermore, glutathione peroxidase activity increased in the submandibular gland adjacent to the apical periodontitis group compared to the control group (p < 0.05), while glutathione, superoxide dismutase, and catalase activities were not altered by apical periodontitis. It is concluded that experimental apical periodontitis alters salivary biochemical composition, in addition to increasing inflammatory markers and inducing local disturbances in the redox state in the parotid and submandibular glands of male rats.
The objective of the study was to evaluate the effects of experimental apical periodontitis on the inflammatory, functional, biochemical, and redox parameters of the parotid and submandibular glands in rats. Twenty 12-week-old male Wistar rats were randomly divided into two groups (n = 10): a control group, without induced lesions and apical periodontitis group, with 4 teeth with induced lesions. After 28 days, pilocarpine stimulated saliva was collected for salivary flow rate and biochemistry composition analysis (total protein, amylase, and electrolytes). The parotid and submandibular glands were sampled for quantification of the tumor necrosis factor-alpha (TNF-α), total oxidative capacity, lipid peroxidation, expressed as thiobarbituric acid-reactive substance, carbonyl protein, total antioxidant capacity, glutathione, superoxide dismutase, catalase, and glutathione peroxidase. Statistical analyses were conducted using the paired Student's t-test (left versus right) and unpaired Student's t-test (control versus apical periodontitis), p < 0.05. TNF-α concentrations were higher in both salivary glands adjacent to the periapical lesions in animals with apical periodontitis (p < 0.05), and also compared to the control group (p < 0.01). Although the salivary flow rate, pH, and buffer capacity were similar between groups, the apical periodontitis group increased the salivary amylase (p < 0.01), chloride (p < 0.05), potassium (p < 0.01), calcium (p < 0.01), and phosphate (p < 0.05). The total oxidant capacity increased in the parotid gland adjacent to the periapical lesions (p < 0.001) in the same rat and compared to the control group (p < 0.05). In contrast, lipid and protein peroxidation did not differ between groups in either salivary gland. Conversely, the total antioxidant capacity of the parotid glands on both sides in the apical periodontitis group was lower than the control group (p < 0.05). Furthermore, glutathione peroxidase activity increased in the submandibular gland adjacent to the apical periodontitis group compared to the control group (p < 0.05), while glutathione, superoxide dismutase, and catalase activities were not altered by apical periodontitis. It is concluded that experimental apical periodontitis alters salivary biochemical composition, in addition to increasing inflammatory markers and inducing local disturbances in the redox state in the parotid and submandibular glands of male rats.
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Palavras-chave
Periodontite apical, Animais Modelo, Saliva, Glândulas salivares, Estresse oxidativo, Proteínas salivares, Salivary glands
Como citar
VAZÃO, A. R. Avaliação da composição salivar e estado redox das glândulas salivares de ratos Wistar portadores de periodontite apical induzida. 2024. 63 f. Dissertação (Mestrado) – Faculdade de Odontologia de Araçatuba, Universidade
Estadual Paulista, Araçatuba, 2024.