L-asparaginase production review: bioprocess design and biochemical characteristics

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dc.contributor.authorCastro, Daniel
dc.contributor.authorMarques, Ana Sofia C.
dc.contributor.authorAlmeida, Mafalda R.
dc.contributor.authorde Paiva, Gabriela B. [UNESP]
dc.contributor.authorBento, Heitor B. S. [UNESP]
dc.contributor.authorPedrolli, Danielle B. [UNESP]
dc.contributor.authorFreire, Mara G.
dc.contributor.authorTavares, Ana P. M.
dc.contributor.authorSantos-Ebinuma, Valéria C. [UNESP]
dc.contributor.institutionUniversity of Aveiro
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:32:07Z
dc.date.available2021-06-25T10:32:07Z
dc.date.issued2021-06-01
dc.description.abstractAbstract: In the past decades, the production of biopharmaceuticals has gained high interest due to its great sensitivity, specificity, and lower risk of negative effects to patients. Biopharmaceuticals are mostly therapeutic recombinant proteins produced through biotechnological processes. In this context, L-asparaginase (L-asparagine amidohydrolase, L-ASNase (E.C. 3.5.1.1)) is a therapeutic enzyme that has been abundantly studied by researchers due to its antineoplastic properties. As a biopharmaceutical, L-ASNase has been used in the treatment of acute lymphoblastic leukemia (ALL), acute myeloblastic leukemia (AML), and other lymphoid malignancies, in combination with other drugs. Besides its application as a biopharmaceutical, this enzyme is widely used in food processing industries as an acrylamide mitigation agent and as a biosensor for the detection of L-asparagine in physiological fluids at nano-levels. The great demand for L-ASNase is supplied by recombinant enzymes from Escherichia coli and Erwinia chrysanthemi. However, production processes are associated to low yields and proteins associated to immunogenicity problems, which leads to the search for a better enzyme source. Considering the L-ASNase pharmacological and food importance, this review provides an overview of the current biotechnological developments in L-ASNase production and biochemical characterization aiming to improve the knowledge about its production. Key points: • Microbial enzyme applications as biopharmaceutical and in food industry • Biosynthesis process: from the microorganism to bioreactor technology • Enzyme activity and kinetic properties: crucial for the final application.en
dc.description.affiliationCICECO-Aveiro Institute of Materials Department of Chemistry University of Aveiro
dc.description.affiliationDepartment of Engineering Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP - São Paulo State University
dc.description.affiliationUnespDepartment of Engineering Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP - São Paulo State University
dc.format.extent4515-4534
dc.identifierhttp://dx.doi.org/10.1007/s00253-021-11359-y
dc.identifier.citationApplied Microbiology and Biotechnology, v. 105, n. 11, p. 4515-4534, 2021.
dc.identifier.doi10.1007/s00253-021-11359-y
dc.identifier.issn1432-0614
dc.identifier.issn0175-7598
dc.identifier.scopus2-s2.0-85107362453
dc.identifier.urihttp://hdl.handle.net/11449/206444
dc.language.isoeng
dc.relation.ispartofApplied Microbiology and Biotechnology
dc.sourceScopus
dc.subjectBiopharmaceutical
dc.subjectBioprocess
dc.subjectFood industry
dc.subjectL-asparaginase
dc.titleL-asparaginase production review: bioprocess design and biochemical characteristicsen
dc.typeResenha
unesp.author.orcid0000-0003-0804-4000[1]
unesp.author.orcid0000-0001-5798-0917[2]
unesp.author.orcid0000-0003-2101-8309[3]
unesp.author.orcid0000-0003-2084-4895[4]
unesp.author.orcid0000-0002-0629-3196[5]
unesp.author.orcid0000-0002-3034-6497[6]
unesp.author.orcid0000-0001-8895-0614[7]
unesp.author.orcid0000-0002-6666-6695[9]

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