5'-ribonucleotides production using 5'-phosphodiesterase from spent malt roots

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dc.contributor.authorAlves, Edson Marcelino [UNESP]
dc.contributor.authorDe Souza, Joyce Faria [UNESP]
dc.contributor.authorMacieja, Szymon [UNESP]
dc.contributor.authorDe Oliva Neto, Pedro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-06-25T10:29:57Z
dc.date.available2021-06-25T10:29:57Z
dc.date.issued2021-01-01
dc.description.abstract5'-ribonucleotides are high value-added molecules widely used in the food and pharmaceutical industries because of their bioactive properties. The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA, and barley malt rootlets as cheap source of 5'-phosphodiesterase (5'-PDE). This is a very promising and innovative strategy because both spent yeast and malt rootles are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. Our results showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the proposed approach can generate a new perspective for the brewing industry regarding the management of its wastes, generating from them products of high added value and with a wide range of applications.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Faculdade de Ciências e Letras de Assis Departamento de Biotecnologia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2016/08886-6
dc.identifierhttp://dx.doi.org/10.1590/1981-6723.24620
dc.identifier.citationBrazilian Journal of Food Technology, v. 24.
dc.identifier.doi10.1590/1981-6723.24620
dc.identifier.fileS1981-67232021000100416.pdf
dc.identifier.issn1981-6723
dc.identifier.scieloS1981-67232021000100416
dc.identifier.scopus2-s2.0-85105427925
dc.identifier.urihttp://hdl.handle.net/11449/206308
dc.language.isopor
dc.relation.ispartofBrazilian Journal of Food Technology
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBarley malt rootlets
dc.subjectBioprocess optimization
dc.subjectBrewing industry wastes
dc.subjectEnzyme kinetics
dc.subjectRNA hydrolysis
dc.subjectSpent brewer’s yeast
dc.title5'-ribonucleotides production using 5'-phosphodiesterase from spent malt rootsen
dc.titleProdução de 5'-ribonucleotídeos utilizando 5'-fosfodiesterase da radícula de malte cervejeiropt
dc.typeArtigo

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