Citral and eugenol modulate DNA damage and pro-inflammatory mediator genes in murine peritoneal macrophages

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dc.contributor.authorPorto, Marilia de Paula [UNESP]
dc.contributor.authorSilva, Glenda Nicioli da
dc.contributor.authorOttoboni Luperini, Bruno Cesar [UNESP]
dc.contributor.authorBachiega, Tatiana Fernanda [UNESP]
dc.contributor.authorCastro Marcondes, Joao Paulo de [UNESP]
dc.contributor.authorSforcin, José Mauricio [UNESP]
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUFOP Univ Fed Ouro Preto
dc.date.accessioned2015-03-18T15:53:04Z
dc.date.available2015-03-18T15:53:04Z
dc.date.issued2014-11-01
dc.description.abstractCitral and eugenol have been broadly studied because of their anti-inflammatory, antioxidant and antiparasitic potentials. In this study, the effects of citral (25, 50 and 100 mu g/mL) and eugenol (0.31, 0.62, 1.24 and 2.48 mu g/mL) on the expression (RT-PCR) of the pro-inflammatory mediator genes NF-kappa B1, COX-2 and TNF-alpha were evaluated in mouse peritoneal macrophages with or without activation by a bacterial lipopolysaccharide (LPS). Additionally, the genotoxic potentials of two compounds and their capacities to modulate the DNA damage induced by doxorubicin (DXR) were investigated using the comet assay. The data revealed that neither citral nor eugenol changed COX-2, NF-kappa B1 or TNF-alpha expression in resting macrophages. However, in LPS-activated cells, citral induced the hypoexpression of COX-2 (100 mu g/mL) and TNF-a (50 and 100 mu g/mL). Hypoexpression of TNF-alpha was also detected after cellular exposure to eugenol at the highest concentration (2.48 mu g/mL). Both compounds exhibited genotoxic potential (citral at 50 and 100 mu g/mL and eugenol at all concentrations) but also showed chemopreventive effects, in various treatment protocols. Both citral and eugenol might modulate inflammatory processes and DXR-induced DNA damage, but the use of these compounds must be viewed with caution because they are also able to induce primary DNA lesions.en
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUFOP Univ Fed Ouro Preto, Escola Farm, Dept Anal Clin, BR-35400000 Ouro Preto, MG, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Med Botucatu, Dept Patol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Imunol, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFUNDUNESP: 01137/10-DFP
dc.description.sponsorshipIdFAPESP: 10/03591-1
dc.format.extent7043-7051
dc.identifierhttp://dx.doi.org/10.1007/s11033-014-3657-9
dc.identifier.citationMolecular Biology Reports. Dordrecht: Springer, v. 41, n. 11, p. 7043-7051, 2014.
dc.identifier.doi10.1007/s11033-014-3657-9
dc.identifier.issn0301-4851
dc.identifier.lattes5051118752980903
dc.identifier.urihttp://hdl.handle.net/11449/116336
dc.identifier.wosWOS:000344101300002
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.ispartofjcr1.889
dc.relation.ispartofsjr0,721
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCitralen
dc.subjectEugenolen
dc.subjectChemopreventionen
dc.subjectDoxorubicinen
dc.subjectGene expressionen
dc.subjectGenotoxicityen
dc.titleCitral and eugenol modulate DNA damage and pro-inflammatory mediator genes in murine peritoneal macrophagesen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0002-5116-2494[5]
unesp.author.orcid0000-0001-9323-3134[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Patologia - FMB
Artigos - Microbiologia e Imunologia - IBB