In Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Function

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dc.contributor.authorBorges, Cibele S.
dc.contributor.authorKiguti, Luiz Ricardo A. [UNESP]
dc.contributor.authorPupo, André S. [UNESP]
dc.contributor.authorKempinas, Wilma G. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUFERSA - Federal Rural University of Semi-Arid Region
dc.date.accessioned2021-06-25T11:10:26Z
dc.date.available2021-06-25T11:10:26Z
dc.date.issued2021-01-01
dc.description.abstractContraction of cauda epididymal duct (CE) smooth muscle is one of the very first events of the seminal emission phase of ejaculation. The contraction of CE smooth muscle is governed by a complex interaction of hormones, autacoids, and by the neurotransmitters released from the epididymal intramural nerve endings, and any impairment in the CE smooth muscle contraction has the potential to impair male fertility. Apart the obvious pathophysiological and toxicological importance of CE smooth muscle contraction, modulation of CE contraction has pharmaceutical interest offering a druggable target to development of drugs to improve/impair male fertility. The in vitro contraction experiments constitute a valuable approach to an in-depth evaluation of functional and molecular changes resulting from pathologies or drug exposure. Therefore, this chapter consists in a description of in vitro pharmacological reactivity contractility of the epididymal duct in a controlled medium, maintained at 30 °C of temperature and continuously bubbled with 95% O2 and 5% CO2 to obtain cumulative concentration–response curves that has been fundamental to some of our investigations on epididymal physiology, toxicology, and pharmacology.en
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences UNESP—São Paulo State University
dc.description.affiliationDepartment of Bioscience - CCBS UFERSA - Federal Rural University of Semi-Arid Region
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences UNESP—São Paulo State University
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences UNESP—São Paulo State University
dc.format.extent65-76
dc.identifierhttp://dx.doi.org/10.1007/978-1-0716-1091-6_6
dc.identifier.citationMethods in Molecular Biology, v. 2240, p. 65-76.
dc.identifier.doi10.1007/978-1-0716-1091-6_6
dc.identifier.issn1940-6029
dc.identifier.issn1064-3745
dc.identifier.scopus2-s2.0-85099690883
dc.identifier.urihttp://hdl.handle.net/11449/208335
dc.language.isoeng
dc.relation.ispartofMethods in Molecular Biology
dc.sourceScopus
dc.subjectContraction
dc.subjectEjaculation
dc.subjectEpididymal duct
dc.subjectMale fertility
dc.subjectSmooth muscle
dc.titleIn Vitro Contraction of Isolated Cauda Epididymal Duct Smooth Muscle as a Complimentary Approach to Physiological, Pathological, Toxicological, and Pharmacological Studies on Epididymal Functionen
dc.typeCapítulo de livro

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Artigos - Morfologia - IBB