Synthesis, characterization and applications of maghemite beads functionalized with rabbit antibodies

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dc.contributor.authorRodriguez, A. F.R.
dc.contributor.authorRocha, C. O. [UNESP]
dc.contributor.authorPiazza, R. D. [UNESP]
dc.contributor.authorDos Santos, C. C. [UNESP]
dc.contributor.authorMorales, M. A.
dc.contributor.authorFaria, F. S.E.D.V.
dc.contributor.authorIqbal, M Zubair
dc.contributor.authorBarbosa, L.
dc.contributor.authorChaves, Y. O.
dc.contributor.authorMariuba, L. A.
dc.contributor.authorJafelicci, M. [UNESP]
dc.contributor.authorMarques, R. F.C. [UNESP]
dc.contributor.institutionLaboratory of Nanobiotechnology
dc.contributor.institutionFederal University of Rio Grande Do Norte
dc.contributor.institutionChinese Academy of Sciences
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:54:27Z
dc.date.available2018-12-11T16:54:27Z
dc.date.issued2018-06-28
dc.description.abstractMagnetic nanoparticles (NPs) have attracted great attention owing to their applications in the biomedical field. In the present work, maghemite (γFe2O3) NPs of 6.5 nm were prepared using a sonochemical method and used to prepare magnetic beads through silanization with 3-aminopropyltrimethoxysilane (APTS). Subsequently, amino groups in the resulting APTS-γFe2O3 beads were converted to carboxylic acid (CARB-γFe2O3) through the succinic anhydride reaction, as confirmed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy and dynamic light scattering (DLS) measurements. The size of these beads was measured as 12 nm and their hydrodynamic diameter as 490 nm, using TEM analysis and DLS, respectively. The CARB-γFe2O3 beads were further functionalized by immobilizing rabbit antibodies on their surfaces; the immobilization was confirmed by flow cytometry and ionic strength. The samples were further characterized by Mössbauer spectroscopy and DC magnetization measurements. Studies on magnetic relaxivities showed that magnetic beads present great potential for application in MR imaging.en
dc.description.affiliationFederal University of Acre Laboratory of Nanobiotechnology
dc.description.affiliationFederal University of Rio Grande Do Norte Department of Theoretical and Experimental Physics
dc.description.affiliationDivision of Functional Materials and Nano-Devices Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences
dc.description.affiliationInstituto Leonidas e Maria Deane Fundacao Oswaldo Cruz
dc.description.affiliationSao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355
dc.description.affiliationUnespSao Paulo State University - UNESP Laboratory of Magnetic Materials and Colloids Institute of Chemistry, Caixa postal 355
dc.identifierhttp://dx.doi.org/10.1088/1361-6528/aacc21
dc.identifier.citationNanotechnology, v. 29, n. 36, 2018.
dc.identifier.doi10.1088/1361-6528/aacc21
dc.identifier.file2-s2.0-85049978755.pdf
dc.identifier.issn1361-6528
dc.identifier.issn0957-4484
dc.identifier.lattes2115942621694174
dc.identifier.orcid0000-0003-0195-3885
dc.identifier.scopus2-s2.0-85049978755
dc.identifier.urihttp://hdl.handle.net/11449/171219
dc.language.isoeng
dc.relation.ispartofNanotechnology
dc.relation.ispartofsjr1,079
dc.relation.ispartofsjr1,079
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectflow cytometry
dc.subjectmagnetic nanobeads
dc.subjectmossbauer spectroscopy
dc.subjectMRI
dc.subjectrabbit antibody
dc.subjectXRD
dc.titleSynthesis, characterization and applications of maghemite beads functionalized with rabbit antibodiesen
dc.typeArtigo
unesp.author.lattes2115942621694174[12]
unesp.author.orcid0000-0002-3034-183X[1]
unesp.author.orcid0000-0003-0195-3885[12]

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