NtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organs

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dc.contributor.authorOtsu, C. T.
dc.contributor.authordaSilva, I
dc.contributor.authorde Molfetta, J. B.
dc.contributor.authorda Silva, L. R.
dc.contributor.authorde Almeida-Engler, J.
dc.contributor.authorEngler, G.
dc.contributor.authorTorraca, P. C.
dc.contributor.authorGoldman, G. H.
dc.contributor.authorGoldman, MHS
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:29:51Z
dc.date.available2014-05-20T15:29:51Z
dc.date.issued2004-08-01
dc.description.abstractTo identify genes specifically or predominantly expressed in the stigmas/styles and to establish their possible function in the reproductive process of plants, a tobacco stigma/style cDNA library was constructed and differentially screened, resulting in the isolation of several cDNA clones. The molecular characterization of one of these clones is described here. After sequencing the cDNA and the isolated genomic clone, it was determined that the corresponding gene encodes a protein containing an ATP-binding cassette, characteristic of ABC transporters. This gene, designated as NtWBC1 (Nicotiana tabacum ABC transporter of the White-Brown Complex subfamily), encodes a protein that contains the typical structure of the 'half-transporters' of the White subfamily. To establish the spatial expression pattern of the NtWBC1 gene, northern blot and real-time RT-PCR analyses with total RNA from roots, stems, leaves, sepals, petals, stamens, stigmas/styles, ovaries, and seeds were performed. The result revealed a transcript of 2.5 kb present at high levels in stigmas and styles and a smaller transcript (2.3 kb) present at a lower level in stamens. NtWBC1 expression is developmentally regulated in stigmas/styles, with mRNA accumulation increasing toward anthesis. In situ hybridization experiments demonstrated that NtWBC1 is expressed in the stigmatic secretory zone and in anthers, at the stomium region and at the vascular bundle. NtWBC1 is the first ABC transporter gene with specific expression in plant reproductive organs to be identified and its expression pattern suggests important role(s) in the reproductive process.en
dc.description.affiliationUniv São Paulo, Dept Biol FFCLRP, BR-14040901 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Genet FMRP, BR-14040900 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, FCAV, BR-14884900 Jaboticabal, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, FCAV, BR-14884900 Jaboticabal, Brazil
dc.format.extent1643-1654
dc.identifierhttp://dx.doi.org/10.1093/jxb/erh195
dc.identifier.citationJournal of Experimental Botany. Oxford: Oxford Univ Press, v. 55, n. 403, p. 1643-1654, 2004.
dc.identifier.doi10.1093/jxb/erh195
dc.identifier.issn0022-0957
dc.identifier.urihttp://hdl.handle.net/11449/39336
dc.identifier.wosWOS:000223348400005
dc.language.isoeng
dc.publisherOxford University Press
dc.relation.ispartofJournal of Experimental Botany
dc.relation.ispartofjcr5.354
dc.relation.ispartofsjr2,822
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectABC transporterpt
dc.subjectdevelopmentally regulated stamen expressionpt
dc.subjectstigma-predominant expressionpt
dc.subjectstigmatic secretory zonept
dc.subjectWhite subfamilypt
dc.titleNtWBC1, an ABC transporter gene specifically expressed in tobacco reproductive organsen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderOxford Univ Press
unesp.author.orcid0000-0002-6786-9320[9]
unesp.author.orcid0000-0002-2986-350X[9]

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