Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: A histological and histometric study in rat calvaria

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dc.contributor.authorGarcia, Valdir Gouveia [UNESP]
dc.contributor.authorDa Conceição, Juliana Mendonça [UNESP]
dc.contributor.authorFernandes, Leandro Araújo [UNESP]
dc.contributor.authorAlmeida, Juliano Milanezi de [UNESP]
dc.contributor.authorNagata, Maria José Hitomi [UNESP]
dc.contributor.authorBosco, Álvaro Francisco [UNESP]
dc.contributor.authorTheodoro, Letícia Helena [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of Alfenas
dc.contributor.institutionBarretos Dental School
dc.date.accessioned2014-05-27T11:28:17Z
dc.date.available2014-05-27T11:28:17Z
dc.date.issued2013-02-01
dc.description.abstractThe purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm2 on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy. © 2012 Springer-Verlag London Ltd.en
dc.description.affiliationDepartment of Surgery and Integrated Clinic Division of Periodontics São Paulo State University (UNESP), Araçatuba, São Paulo
dc.description.affiliationDepartment of Clinic and Surgery Federal University of Alfenas, Alfenas, Minas Gerais
dc.description.affiliationDepartment of Periodontics University Center of the Educational Foundation of Barretos (UNIFEB) Barretos Dental School, Barretos, São Paulo
dc.description.affiliationUnespDepartment of Surgery and Integrated Clinic Division of Periodontics São Paulo State University (UNESP), Araçatuba, São Paulo
dc.format.extent407-414
dc.identifierhttp://dx.doi.org/10.1007/s10103-012-1068-5
dc.identifier.citationLasers in Medical Science, v. 28, n. 2, p. 407-414, 2013.
dc.identifier.doi10.1007/s10103-012-1068-5
dc.identifier.issn0268-8921
dc.identifier.issn1435-604X
dc.identifier.lattes4068921369233125
dc.identifier.lattes8399870097572073
dc.identifier.lattes9831236034935598
dc.identifier.lattes4774447118127346
dc.identifier.scopus2-s2.0-84879689491
dc.identifier.urihttp://hdl.handle.net/11449/74451
dc.identifier.wosWOS:000315579400008
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofjcr1.949
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAlendronate
dc.subjectBisphosphonate
dc.subjectBone regeneration
dc.subjectLaser therapy
dc.subjectRats
dc.subjectalendronic acid
dc.subjectbisphosphonic acid derivative
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectanimal tissue
dc.subjectbone defect
dc.subjectbone regeneration
dc.subjectcalvaria
dc.subjectcontrolled study
dc.subjectdisease severity
dc.subjectexperimental study
dc.subjectfemale
dc.subjectfracture healing
dc.subjecthistometry
dc.subjecthistopathology
dc.subjectlow level laser therapy
dc.subjectnonhuman
dc.subjectossification
dc.subjectosteopenia
dc.subjectpriority journal
dc.subjectrat
dc.subjecttherapy effect
dc.subjecttreatment response
dc.subjectWistar rat
dc.titleEffects of LLLT in combination with bisphosphonate on bone healing in critical size defects: A histological and histometric study in rat calvariaen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.lattes4068921369233125
unesp.author.lattes8399870097572073
unesp.author.lattes9831236034935598
unesp.author.lattes4774447118127346
unesp.author.orcid0000-0002-6715-8334[1]
unesp.author.orcid0000-0001-5903-5652[5]
unesp.author.orcid0000-0002-5995-5747[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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