Efficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milk

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dc.contributor.authorSilva, Higor Oliveira [UNESP]
dc.contributor.authorSantos Lima, Joyce Aparecida [UNESP]
dc.contributor.authorGamero Aguilar, Carlos Eduardo [UNESP]
dc.contributor.authorMarques Rossi, Gabriel Augusto [UNESP]
dc.contributor.authorMathias, Luis Antonio [UNESP]
dc.contributor.authorCentola Vidal, Ana Maria
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-05T04:09:56Z
dc.date.available2019-10-05T04:09:56Z
dc.date.issued2018-11-28
dc.description.abstractThe species of the Bacillus cereus group have the ability to adhere to and form biofilms on solid surfaces, including stainless steel, a material widely used in food industries. Biofilms allow for recontamination during food processing, and the clean-in-place (CIP) system is largely used by industries to control them. This study thus proposes to evaluate the efficacy of peracetic acid and sodium hypochlorite against biofilms induced on stainless-steel surfaces. The SAMN07414939 isolate (BioProject PRJNA390851), a recognized biofilm producer, was selected for biofilm induction on AISI 304 stainless steel. Biofilm induction was performed and classified into three categories: TCP (Tindalized, Contaminated, and Pasteurized milk), TCS (Tindalized milk Contaminated with Spores), and TCV (Tindalized milk Contaminated with Vegetative cells). Subsequently, the coupons were sanitized simulating a CIP procedure, on a pilot scale, using alkaline and acid solutions followed by disinfectants (peracetic acid and sodium hypochlorite). Microorganism adhesion on the surfaces reached 6.3 x 10(5) to 3.1 x 10(7) CFU/cm(-2). Results did not show significant differences (p > 0.05) for surface adhesion between the three tested categories (TOP, TCS, and TCV) or (p > 0.05) between the two disinfectants (peracetic acid and sodium hypochlorite). Microbial populations adhered to the stainless-steel coupons are equally reduced after treatment with peracetic acid and sodium hypochlorite, with no differences in the control of B. cereus s.s. biofilms on AISI 304 stainless-steel surfaces.en
dc.description.affiliationSao Paulo State Univ, Sch Agrarian & Veterinarian Sci, Dept Prevent Vet Med & Anim Reprod, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Dept Vet Med, Sch Anim Sci & Food Engn, Sao Paulo, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Sch Agrarian & Veterinarian Sci, Dept Prevent Vet Med & Anim Reprod, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNational Council for Scientific and Technological Development
dc.description.sponsorshipIdFAPESP: 2015/20874-0
dc.description.sponsorshipIdNational Council for Scientific and Technological Development: 2014/166512-1
dc.format.extent11
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2018.02934
dc.identifier.citationFrontiers In Microbiology. Lausanne: Frontiers Media Sa, v. 9, 11 p., 2018.
dc.identifier.doi10.3389/fmicb.2018.02934
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/11449/186509
dc.identifier.wosWOS:000451644300001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectperacetic acid
dc.subjectsodium hypochlorite
dc.subjectrecontamination
dc.subjectfood safety
dc.subjectbiofilm formation
dc.titleEfficiency of Different Disinfectants on Bacillus cereus Sensu Stricto Biofilms on Stainless-Steel Surfaces in Contact With Milken
dc.typeArtigo
dcterms.rightsHolderFrontiers Media Sa

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