Comparison of the properties of compacted and porous lamellar chitosan-xanthan membranes as dressings and scaffolds for the treatment of skin lesions

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dc.contributor.authorBellini, Marcia Zilioli
dc.contributor.authorResende Pires, Ana Luiza [UNESP]
dc.contributor.authorVasconcelos, Mariana Oliveira [UNESP]
dc.contributor.authorMoraes, Angela Maria
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:22:12Z
dc.date.available2014-05-20T13:22:12Z
dc.date.issued2012-09-25
dc.description.abstractCompacted and porous lamellar membranes of xanthan (Xn) and chitosan (Ch) at mass ratios of 1 : 1 and 1.2 : 0.8 were prepared and tested to verify possible applications in the treatment of skin lesions. All membranes were prepared by complexation of the polysaccharides in solution and subsequent casting. The porous membranes were obtained by adding either Tween 80 or Pluronic F68 to the polysaccharide complexes before casting. Membranes prepared in the absence of surfactants at a mass ratio of Xn to Ch of 1 : 1 proved ideal for use as wound dressings, as they were thin (around 0.10 mm in thickness) and transparent and had low in vitro cytotoxicity to L929 cells, a tensile strength at break of 25 MPa, water absorption after 24 h of around 86 g/g and simulated body fluid absorption of 16% and adequate stability in the presence of the same solutions. Membranes prepared at the mass ratio of Xn to Ch of 1 : 1 in the presence of Pluronic F68 showed the most favorable characteristics for application as scaffolds for tissue engineering. These membranes consisted of a matrix with interconnected pores which were distributed homogeneously throughout the structure and had a thickness of 1.84 mm, high capacity for FBS uptake (around 18 g/g) and cell culture medium uptake (8.6 g/g), a loss of mass in the culture medium of 33% after 144 h, and low in vitro toxicity to L929 cells. In conclusion, membranes of Ch and Xn produced in the presence or absence of the surfactant Pluronic F68 have a high potential for use as scaffolds in tissue engineering or as dermal dressings, respectively, whereas in contrast, membranes prepared in the presence of Tween 80, regardless of the mass ratio of Xn to Ch, were very cytotoxic to L929 cells and therefore were not appropriate for any of the proposed applications. (C) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012en
dc.description.affiliationUniv Campinas UNICAMP, Sch Chem Engn, Dept Engn Mat & Bioproc, BR-13083852 Campinas, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Biol Sci, BR-19806900 Assis, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extentE421-E431
dc.identifierhttp://dx.doi.org/10.1002/app.36693
dc.identifier.citationJournal of Applied Polymer Science. Hoboken: Wiley-blackwell, v. 125, p. E421-E431, 2012.
dc.identifier.doi10.1002/app.36693
dc.identifier.issn0021-8995
dc.identifier.urihttp://hdl.handle.net/11449/6495
dc.identifier.wosWOS:000304862900051
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Polymer Science
dc.relation.ispartofjcr1.901
dc.relation.ispartofsjr0,543
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectchitosanen
dc.subjectxanthanen
dc.subjectmembraneen
dc.subjectwound dressingen
dc.subjectscaffolden
dc.subjecttissue engineeringen
dc.titleComparison of the properties of compacted and porous lamellar chitosan-xanthan membranes as dressings and scaffolds for the treatment of skin lesionsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0002-5813-332X[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt

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