Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

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dc.contributor.authorOliveira, GH [UNESP]
dc.contributor.authorBerchieri Jr, A [UNESP]
dc.contributor.authorMontassier, Helio Jose [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:15:55Z
dc.date.available2014-05-20T13:15:55Z
dc.date.issued2006-03-01
dc.description.abstractThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.en
dc.description.affiliationUNESP FCAV Department of Animal Pathology
dc.description.affiliationUnespUNESP FCAV Department of Animal Pathology
dc.format.extent51-54
dc.identifierhttp://dx.doi.org/10.1590/S1516-635X2006000100008
dc.identifier.citationRevista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.
dc.identifier.doi10.1590/S1516-635X2006000100008
dc.identifier.fileS1516-635X2006000100008.pdf
dc.identifier.issn1516-635X
dc.identifier.scieloS1516-635X2006000100008
dc.identifier.urihttp://hdl.handle.net/11449/2927
dc.language.isoeng
dc.publisherFundação APINCO de Ciência e Tecnologia Avícolas
dc.relation.ispartofRevista Brasileira de Ciência Avícola
dc.relation.ispartofjcr0.463
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectChickenen
dc.subjectELISAen
dc.subjectenzymeen
dc.subjectSalmonella spen
dc.subjectSerologyen
dc.titleChicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisaen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Patologia Veterinária - FCAV