Attraction of the cutaneous leishmaniasis vector Nyssomyia neivai (Diptera: Psychodidae) to host odour components in a wind tunnel

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dc.contributor.authorPinto, Mara Cristina [UNESP]
dc.contributor.authorBray, Daniel P.
dc.contributor.authorEiras, Alvaro E.
dc.contributor.authorCarvalheira, Henrique P. [UNESP]
dc.contributor.authorPuertas, Camila P. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionKeele Univ
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2014-05-20T13:24:21Z
dc.date.available2014-05-20T13:24:21Z
dc.date.issued2012-09-25
dc.description.abstractBackground: Laboratory studies of host-seeking olfactory behaviour in sandflies have largely been restricted to the American visceral leishmaniasis vector Lutzomyia longipalpis. In comparison, almost nothing is known about the chemical ecology of related species, which transmit American cutaneous leishmaniasis (ACL), due in part to difficulties in raising these insects in the laboratory. Understanding how ACL vectors locate their hosts will be essential to developing new vector control strategies to combat this debilitating disease.Methods: This study examined host-odour seeking behaviour of the ACL vector Nyssomyia neivai (Pinto) (=Lutzomyia neivai) using a wind tunnel olfactometer. The primary aim was to determine whether field-collected female N. neivai would respond to host odours in the laboratory, thereby eliminating the need to maintain colonies of these insects for behavioural experiments. Responses to two key host odour components, 1-octen-3-ol and lactic acid, and a commercially-available mosquito lure (BG-Lure (TM)) were assessed and compared relative to an air control. We also tested whether trials could be conducted outside of the normal evening activity period of N. neivai without impacting on fly behaviour, and whether the same flies could be used to assess baseline responses to air without affecting responses to octenol, thereby reducing the number of flies required for experiments.Results: Octenol was found to both activate host-seeking behaviour and attract female N. neivai in the wind tunnel, while lactic acid elicited weaker responses of activation and attractiveness under identical conditions. The BG-Lure did not activate or attract N. neivai under test conditions. Further experiments showed that sandfly behaviour in the wind tunnel was not affected by time of day, such that experiments need not be restricted to nocturnal hours. Moreover, using the same flies to measure both baseline responses to air and attraction to test compounds did not affect odour-seeking behaviour.Conclusions: The results of this study demonstrate that N. neivai taken from the field are suitable for use in laboratory olfactometer experiments. It is hoped this work will facilitate further research into chemical ecology of this species, and other ACL vectors.en
dc.description.affiliationUniv Estadual Julio de Mesquita Filho, Parasitol Lab, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationKeele Univ, Inst Sci & Technol Med, Chem Ecol Grp, Keele ST5 5BG, Staffs, England
dc.description.affiliationUniversidade Federal de Minas Gerais (UFMG), Lab Ecol Quim Insetos Vetores, Dept Parasitol, Inst Ciencias Biol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUnespUniv Estadual Julio de Mesquita Filho, Parasitol Lab, Dept Ciencias Biol, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipInternational Foundation for Science (IFS)
dc.description.sponsorshipWellcome Trust
dc.description.sponsorshipIdIFS: F-4587-1
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1186/1756-3305-5-210
dc.identifier.citationParasites & Vectors. London: Biomed Central Ltd., v. 5, p. 8, 2012.
dc.identifier.doi10.1186/1756-3305-5-210
dc.identifier.fileWOS000310460200001.pdf
dc.identifier.issn1756-3305
dc.identifier.lattes8221737491598415
dc.identifier.urihttp://hdl.handle.net/11449/7517
dc.identifier.wosWOS:000310460200001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofParasites & Vectors
dc.relation.ispartofjcr3.163
dc.relation.ispartofsjr1,702
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectKairomoneen
dc.subjectOctenolen
dc.subjectLactic aciden
dc.subjectAmmoniaen
dc.subjectCaproic aciden
dc.subjectBG Lureen
dc.subjectSandfliesen
dc.subjectVector controlen
dc.subjectWind tunnelen
dc.titleAttraction of the cutaneous leishmaniasis vector Nyssomyia neivai (Diptera: Psychodidae) to host odour components in a wind tunnelen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes8221737491598415
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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