Staphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genes

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dc.contributor.authorPinheiro, Luiza [UNESP]
dc.contributor.authorBrito, Carla Ivo [UNESP]
dc.contributor.authorOliveira, Adilson de [UNESP]
dc.contributor.authorMartins, Patrícia Yoshida Faccioli [UNESP]
dc.contributor.authorPereira, Valéria Cataneli [UNESP]
dc.contributor.authorCunha, Maria de Lourdes Ribeiro de Souza da [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:32:51Z
dc.date.available2015-12-07T15:32:51Z
dc.date.issued2015
dc.description.abstractAlthough opportunistic pathogens, coagulase-negative staphylococci (CoNS), including Staphylococcus epidermidis and Staphylococcus haemolyticus, have long been regarded as avirulent organisms. The role of toxins in the development of infections caused by CoNS is still controversial. The objective of this study was to characterize the presence of enterotoxin and cytotoxin genes in S. epidermidis and S. haemolyticus isolates obtained from blood cultures. Cytotoxin genes were detected by PCR using novel species-specific primers. Among the 85 S. epidermidis and 84 S. haemolyticus isolates, 95.3% and 79.8%, respectively, carried at least one enterotoxin gene. The most frequent enterotoxin genes were sea (53.3%), seg (64.5%) and sei (67.5%). The seg gene was positively associated with S. epidermidis (p = 0.02), and this species was more toxigenic than S. haemolyticus. The hla/yidD gene was detected in 92.9% of S. epidermidis and the hla gene in 91.7% of S. haemolyticus isolates; hlb was detected in 92.9% of the S. epidermidis isolates and hld in 95.3%. Nosocomial Staphylococcus epidermidis and S. haemolyticus isolates exhibited a high toxigenic potential, mainly producing the non-classical enterotoxins seg and sei. The previously unreported detection of hla/yidD and hlb in S. epidermidis and S. haemolyticus using species-specific primers showed that these hemolysin genes differ between CoNS species and that they are highly frequent in blood culture isolates.en
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Microbiologia e Imunologia, Instituto de Biociências de Botucatu
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2011/15396-1
dc.description.sponsorshipIdCNPq: 304729/2014-0
dc.format.extent3688-3699
dc.identifierhttp://dx.doi.org/10.3390/toxins7093688
dc.identifier.citationToxins, v. 7, n. 9, p. 3688-3699, 2015.
dc.identifier.doi10.3390/toxins7093688
dc.identifier.filePMC4591658.pdf
dc.identifier.issn2072-6651
dc.identifier.lattes0115647772315973
dc.identifier.pmcPMC4591658
dc.identifier.pubmed26389954
dc.identifier.urihttp://hdl.handle.net/11449/131230
dc.language.isoeng
dc.relation.ispartofToxins
dc.relation.ispartofjcr3.273
dc.relation.ispartofsjr0,955
dc.rights.accessRightsAcesso aberto
dc.sourcePubMed
dc.subjectStaphylococcus epidermidisen
dc.subjectStaphylococcus haemolyticusen
dc.subjectCytotoxinsen
dc.subjectEnterotoxinsen
dc.titleStaphylococcus epidermidis and Staphylococcus haemolyticus: molecular detection of cytotoxin and enterotoxin genesen
dc.typeArtigo
unesp.author.lattes0115647772315973
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB