Abdominal hyperalgesia in secretory phospholipase A(2)-induced rat pancreatitis: Distinct roles of NK1 receptors

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dc.contributor.authorCamargo, Enilton A.
dc.contributor.authorZanoni, Cristiane I.
dc.contributor.authorToyama, Marcos H. [UNESP]
dc.contributor.authorMuscara, Marcelo N.
dc.contributor.authorDocherty, Reginald J.
dc.contributor.authorCosta, Soraia K. P.
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionKings Coll London
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:21Z
dc.date.available2014-05-20T13:12:21Z
dc.date.issued2011-10-01
dc.description.abstractWe investigated the potential of secretory phospholipase A(2) (sPLA(2))-induced pancreatitis to promote abdominal hyperalgesia, as well as to depolarize sensory fibres in vitro using a grease-gap technique. Pancreatitis was induced by the injection of sPLA(2) from Crotalus durissus terrificus (sPLA(2) Cdt, 300 mu g kg(-1)) venom into the common bile duct of rats. Pancreatic inflammatory signs, serum amylase levels and abdominal hyperalgesia were evaluated in rats treated or not with SR140333, a tachykinin NK1 receptor antagonist. Injection of sPLA(2) Cdt caused pancreatic oedema formation and increased pancreatic neutrophil infiltration and serum amylase at 4 h, which returned to normality by 24 h, except for the neutrophil infiltration, which was still increased at this time point. Animals injected with sPLA(2) exhibited a lower withdrawal threshold to electronic von Frey stimulation in the upper abdominal region at 4 h, but not 24 h, post-injection when compared with saline-injected rats. Pre-treatment of animals with SR140333 significantly reduced the sPLA(2) Cdt-induced abdominal hyperalgesia, without affecting the other parameters. Neither sPLA(2) Cdt nor sPLA(2) from Naja mocambique mocambique venom depolarized capsaicin-sensitive sensory fibres from rat vagus nerve, but they decreased the propagated compound action potentials in both A and C fibres. These data show for the first time that NK1 receptors play an important role in the early abdominal hyperalgesia in a rat model of sPLA(2)-induced pancreatitis, suggesting that these receptors are of importance in the development of pain in the pancreatitis condition. We also provide evidence that sPLA(2)s do not directly depolarize sensory fibres in vitro. (C) 2011 European Federation of International Association for the Study of Pain Chapters. Published by Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sergipe, Dept Physiol, BR-49100000 Aracaju, SE, Brazil
dc.description.affiliationUniv São Paulo, Inst Biosci ICB I, Dept Pharmacol, BR-05508900 São Paulo, Brazil
dc.description.affiliationKings Coll London, Sch Biomed & Hlth Sci, Wolfson Ctr Age Related Dis, London SE1 9RT, England
dc.description.affiliationUniv São Paulo State UNESP, Sao Vicente Unit, São Paulo, Brazil
dc.description.affiliationUnespUniv São Paulo State UNESP, Sao Vicente Unit, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 07/00529-0
dc.description.sponsorshipIdFAPESP: 06/61591-2
dc.description.sponsorshipIdCNPq: 476416/2009-4
dc.format.extent900-906
dc.identifierhttp://dx.doi.org/10.1016/j.ejpain.2011.04.001
dc.identifier.citationEuropean Journal of Pain. Oxford: Elsevier B.V., v. 15, n. 9, p. 900-906, 2011.
dc.identifier.doi10.1016/j.ejpain.2011.04.001
dc.identifier.issn1090-3801
dc.identifier.urihttp://hdl.handle.net/11449/332
dc.identifier.wosWOS:000296388900004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofEuropean Journal of Pain
dc.relation.ispartofjcr2.991
dc.relation.ispartofsjr1,380
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAcute pancreatitisen
dc.subjectPhospholipase A(2)en
dc.subjectAbdominal hyperalgesiaen
dc.subjectNeurokinin-1 receptoren
dc.subjectVagus nerveen
dc.titleAbdominal hyperalgesia in secretory phospholipase A(2)-induced rat pancreatitis: Distinct roles of NK1 receptorsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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Artigos - Ciências Biológicas - São Vicente