Cat ovarian follicle ultrastructure after cryopreservation with ethylene glycol and dimethyl sulfoxide

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dc.contributor.authorLeonel, Ellen Cristina Rivas [UNESP]
dc.contributor.authorVilela, Janice Miranda Vasconcellos
dc.contributor.authorCarrilho, Daniela de Jesus
dc.contributor.authorLucci, Carolina Madeira
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:21:20Z
dc.date.available2018-12-11T17:21:20Z
dc.date.issued2018-08-01
dc.description.abstractOvarian tissue cryopreservation is a promising technique for fertility maintenance. The aim of this study was to compare the morphology of domestic cat ovarian follicles after tissue cryopreservation with ethylene glycol (EG) and dimethyl sulfoxide (Me2SO). Ovaries from healthy adult cats undergoing elective ovariohysterectomy were used. Eight fragments were obtained from each pair of ovaries: two were used as fresh controls; three were submitted to fresh perfusion toxicity test and perfused with M199, 10% fetal calf serum and 0.4% sucrose containing Me2SO 1.5 M, EG 1.5 M or Me2SO 0.75 M + EG 0.75 M; and the remaining three fragments were perfused as described and submitted to slow freezing. After 45 days of cryopreservation, the samples were thawed, fixed and processed for light and transmission electron microscopy (TEM). The percentages of morphologically normal follicles identified by light microscopy were higher in the control group (94.45%) in comparison to the frozen groups (80.56% with EG, 78.7% with Me2SO and 75.87% with EG + Me2SO). The fresh perfused tissue showed no statistical difference compared to control or frozen samples. The TEM analysis showed less damage in the ultrastructure of follicles from the Me2SO group in comparison with the EG and Me2SO + EG groups. According to the morphological analysis, 1.5 M Me2SO is the best cryoprotectant for cryopreservation of domestic cat ovarian tissue regarding the morphology of preantral follicles after thawing. Further studies regarding the viability of these follicles should be performed.en
dc.description.affiliationDepartamento de Ciências Fisiológicas Instituto de Ciências Biológicas Universidade de Brasília Campus Universitário Darcy Ribeiro, Asa Norte
dc.description.affiliationDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265, Jardim Nazareth
dc.description.affiliationUnespDepartamento de Biologia Instituto de Biociências Letras e Ciências Exatas (IBILCE) Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265, Jardim Nazareth
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent9-14
dc.identifierhttp://dx.doi.org/10.1016/j.cryobiol.2018.07.003
dc.identifier.citationCryobiology, v. 83, p. 9-14.
dc.identifier.doi10.1016/j.cryobiol.2018.07.003
dc.identifier.file2-s2.0-85049491057.pdf
dc.identifier.issn1090-2392
dc.identifier.issn0011-2240
dc.identifier.scopus2-s2.0-85049491057
dc.identifier.urihttp://hdl.handle.net/11449/176554
dc.language.isoeng
dc.relation.ispartofCryobiology
dc.relation.ispartofsjr0,836
dc.relation.ispartofsjr0,836
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCryoprotectant
dc.subjectFeline
dc.subjectMe2SO
dc.subjectOvarian follicle
dc.subjectOvary
dc.titleCat ovarian follicle ultrastructure after cryopreservation with ethylene glycol and dimethyl sulfoxideen
dc.typeArtigo
unesp.author.orcid0000-0003-1840-9974[2]

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