The negligible effects of the antifungal natamycin on cholesterol-dipalmitoyl phosphatidylcholine monolayers may explain its low oral and topical toxicity for mammals

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dc.contributor.authorArima, Anderson A. [UNESP]
dc.contributor.authorPavinatto, Felippe J.
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.authorGonzales, Eduardo R. P. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2015-03-18T15:53:23Z
dc.date.available2015-03-18T15:53:23Z
dc.date.issued2014-10-01
dc.description.abstractNatamycin is an effective, broad spectrum antifungal with no reported resistance, in contrast to most antimicrobials. It also exhibits reduced (oral and topical) toxicity to humans, which is probably associated with the lack of effects on mammalian cell membranes. In this paper we employ Langmuir monolayers to mimic a cell membrane, whose properties are interrogated with various techniques. We found that natamycin has negligible effects on Langmuir monolayers of dipalmitoyl phosphatidylcholine (DPPC), but it strongly affects cholesterol monolayers. Natamycin causes the surface pressure isotherm of a cholesterol monolayer to expand even at high surface pressures since it penetrates into the hydrophobic chains. It also reduces the compressibility modulus, probably because natamycin disturbs the organization of the cholesterol molecules, as inferred with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In mixed cholesterol/DPPC monolayers, strong effects from natamycin were only observed when the cholesterol concentration was 50 mol% or higher, well above its concentration in a mammalian cell membrane. For a sterol concentration that mimics a real cell membrane in mammals, i.e. with 25 mol% of cholesterol, the effects were negligible, which may explain why natamycin has low toxicity when ingested and/or employed to treat superficial fungal infections. (C) 2014 Elsevier B.V. All rights reserved.en
dc.description.affiliationState Univ Sao Paulo, Fac Sci & Technol, Presidente Prudente, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Sao Carlos Inst Phys, Sao Carlos, SP, Brazil
dc.description.affiliationUnespState Univ Sao Paulo, Fac Sci & Technol, Presidente Prudente, SP, Brazil
dc.description.sponsorshipPOSMAT
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipnBioNet network (Brazil)
dc.format.extent202-208
dc.identifierhttp://dx.doi.org/10.1016/j.colsurfb.2014.06.058
dc.identifier.citationColloids And Surfaces B-biointerfaces. Amsterdam: Elsevier Science Bv, v. 122, p. 202-208, 2014.
dc.identifier.doi10.1016/j.colsurfb.2014.06.058
dc.identifier.issn0927-7765
dc.identifier.urihttp://hdl.handle.net/11449/116487
dc.identifier.wosWOS:000343612900026
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofColloids And Surfaces B-biointerfaces
dc.relation.ispartofjcr3.997
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectNatamycinen
dc.subjectCholesterolen
dc.subjectDipalmitoyl phosphatidylcholine (DPPC)en
dc.subjectLangmuir filmsen
dc.titleThe negligible effects of the antifungal natamycin on cholesterol-dipalmitoyl phosphatidylcholine monolayers may explain its low oral and topical toxicity for mammalsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-5399-5860[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

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