Evaluation of the Integrity of Horse Hoof Dermal and Epidermal Tissues Collected by Dorsal Transmural Access

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dc.contributor.authorGravena, Kamila [UNESP]
dc.contributor.authorde Lima Sampaio, Rita de Cassia [UNESP]
dc.contributor.authorMartins Dias, Deborah Penteado [UNESP]
dc.contributor.authorCanola, Paulo Alescio [UNESP]
dc.contributor.authorPeiro, Juliana Regina [UNESP]
dc.contributor.authorde Lacerda-Neto, Jose Correa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:14:16Z
dc.date.available2014-05-20T13:14:16Z
dc.date.issued2012-12-01
dc.description.abstractThe purpose of this study was to evaluate the integrity of dermal and epidermal tissues collected by dorsal transmural access. Eight healthy adult horses were subjected to two biopsies of the right hoof, with a 24-hour interval. To ensure hoof integrity, a radiographic evaluation with dorsopalmar and lateromedial positions was performed. The animals were sedated, and the pedal tissue was collected from two sites that were 2 cm distal to the coronary band. For each biopsy, the hoof wall was perforated using an aluminum oxide drill bit, 4.8 mm in diameter that was connected to a drilling machine, yielding a hole of approximately 9.0 mm. The perforation procedure was performed slowly in the stratum medium until 1 mm of it remained, which was determined by evaluating the movement in response to pressure applied with a Halstead forceps. Laminar deep incisions were performed with a number 11 scalpel blade until the distal phalanx was reached. The laminar tissue was then removed using a Frahm scaler. A cuboid laminar sample measuring approximately 5 mm at the base and 7 mm in height was obtained. The samples were suitable for analysis by histology and transmission electron microscopy showing that the ends of the secondary epidermal laminae were round, the basement membrane was intact between the secondary epidermal laminae and secondary dermal laminae, and the ultrastructural analysis of the cells showed their structural integrity. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Dept Clin & Cirurgia Vet, FCAV, BR-14884900 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Fac Odontol & Med Vet, FOA, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin & Cirurgia Vet, FCAV, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Clin Cirurgia & Reprod Anim, Fac Odontol & Med Vet, FOA, BR-14884900 São Paulo, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent858-862
dc.identifierhttp://dx.doi.org/10.1016/j.jevs.2012.03.018
dc.identifier.citationJournal of Equine Veterinary Science. New York: Elsevier B.V., v. 32, n. 12, p. 858-862, 2012.
dc.identifier.doi10.1016/j.jevs.2012.03.018
dc.identifier.issn0737-0806
dc.identifier.lattes3874036760952509
dc.identifier.orcid0000-0003-4354-6487
dc.identifier.urihttp://hdl.handle.net/11449/1792
dc.identifier.wosWOS:000312362100016
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Equine Veterinary Science
dc.relation.ispartofjcr0.880
dc.relation.ispartofsjr0,390
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHorseen
dc.subjectHistologyen
dc.subjectLaminaeen
dc.subjectPedal biopsyen
dc.subjectPodiatryen
dc.titleEvaluation of the Integrity of Horse Hoof Dermal and Epidermal Tissues Collected by Dorsal Transmural Accessen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3874036760952509[4]
unesp.author.orcid0000-0002-5659-0404[6]
unesp.author.orcid0000-0003-4354-6487[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Clínica e Cirurgia Veterinária - FCAV