Periodontal Tissue Engineering After Tooth Replantation

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dc.contributor.authorda Silva Assuncao, Luciana Reichert
dc.contributor.authorColenci, Renato [UNESP]
dc.contributor.authorFerreira do-Amaral, Caril Constante [UNESP]
dc.contributor.authorSonoda, Celso Koogi [UNESP]
dc.contributor.authorBomfim, Suely Regina Mogami [UNESP]
dc.contributor.authorOkamoto, Roberta [UNESP]
dc.contributor.authorGolim, Márjorie de Assis [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorPercinoto, Célio [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.institutionUniversidade Federal do Pará (UFPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:29:45Z
dc.date.accessioned2014-05-20T13:43:26Z
dc.date.available2013-09-30T18:29:45Z
dc.date.available2014-05-20T13:43:26Z
dc.date.issued2011-05-01
dc.description.abstractBackground: Blood-derived products, platelet-poor plasma (PPP) and platelet-rich plasma (PRP), constitute an approach in the enhancement of tissue healing. PRP has also been used as a scaffold for bone marrow stem cells in tissue engineering. This study evaluates the effect of PPP, calcium chloride activated PRP (PRP/Ca), calcium chloride and thrombin-activated PRP (PRP/Thr/Ca), and bone marrow mononuclear cells and PRP/Ca (BMMCs/PRP/Ca) on the healing of replanted dog teeth.Methods: After 30 minutes of extraction, teeth were replanted with 1) no material (control); 2) PPP; 3) PRP/Ca; 4) PRP/Thr/Ca; or 5) BMMCs/PRP/Ca. Histologic, histomorphometric, and immunohistochemical analysis was assessed 120 days after replantation. Data from histomorphometric analysis were analyzed statistically (analysis of variance, Tukey; P < 0.05). Quantitative immunohistochemical analysis was analyzed by Kruskal-Wallis and Dunn post hoc test (P < 0.05).Results: Flow cytometry analysis showed 55.98% of CD34(+) and 32.67% of CD90/Thy-1 for BMMCs sample. BMMCs/PRP/Ca presented the largest areas of replacement resorption characterized by osseous ingrowth into cementum (P < 0.05), with intense immunomarcation for tartrate-resistant acid phosphatase. The PRP/Ca group also showed areas of replacement resorption with significant immunomarcation for osteopontin. PRP/Thr/Ca presented no replacement resorption. PPP showed areas of inflammatory resorption, with immunomarcation for tartrate-resistant acid phosphatase.Conclusions: The results suggest that platelets activated with thrombin play an important role in the healing of tissues after tooth replantation. Additional studies are necessary to test other materials, because PRP/Ca did not present an appropriate scaffold for undifferentiated cells in the treatment of avulsed teeth. J Periodontol 2011;82:758-766.en
dc.description.affiliationFed Univ Para UFPA, Sch Dent, BR-66075900 Belem, Para, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Med, Lab Cellular Engn, Botucatu Blood Ctr, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent, Dept Basic Sci, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent, Dept Surg & Integrated Clin, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent, Dept Clin & Surg & Anim Reprod, Vet Med Course, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Fac Med, Botucatu Blood Ctr, Lab Flow Cytometry, São Paulo, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Sch Dent, Dept Pediat Dent, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Med, Lab Cellular Engn, Botucatu Blood Ctr, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent, Dept Basic Sci, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent, Dept Surg & Integrated Clin, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent, Dept Clin & Surg & Anim Reprod, Vet Med Course, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Fac Med, Botucatu Blood Ctr, Lab Flow Cytometry, São Paulo, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Sch Dent, Dept Pediat Dent, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 06/59420-5
dc.format.extent758-766
dc.identifierhttp://dx.doi.org/10.1902/jop.2010.100448
dc.identifier.citationJournal of Periodontology. Chicago: Amer Acad Periodontology, v. 82, n. 5, p. 758-766, 2011.
dc.identifier.doi10.1902/jop.2010.100448
dc.identifier.issn0022-3492
dc.identifier.lattes9646764071339214
dc.identifier.lattes5007703439443544
dc.identifier.lattes1566928219828056
dc.identifier.urihttp://hdl.handle.net/11449/15151
dc.identifier.wosWOS:000290414700015
dc.language.isoeng
dc.publisherAmer Acad Periodontology
dc.relation.ispartofJournal of Periodontology
dc.relation.ispartofjcr3.392
dc.relation.ispartofsjr1,408
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBone marrowen
dc.subjectplatelet-rich plasmaen
dc.subjectstem cellsen
dc.subjecttissue engineeringen
dc.subjecttooth replantationen
dc.titlePeriodontal Tissue Engineering After Tooth Replantationen
dc.typeArtigo
dcterms.licensehttp://www.joponline.org/page/permissionRequests.jsp
dcterms.rightsHolderAmer Acad Periodontology
unesp.author.lattes1566928219828056
unesp.author.lattes5007703439443544
unesp.author.lattes9646764071339214
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Cirurgia e Clínica Integrada - FOA
Artigos - Ciências Básicas - FOA
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