Effect of diamond-like carbon thin film coated acrylic resin on candida albicans biofilm formation

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dc.contributor.authorQueiroz, José Renato Cavalcanti
dc.contributor.authorFissmer, Sara Fernanda
dc.contributor.authorKoga-Ito, Cristiane Yumi [UNESP]
dc.contributor.authorSalvia, Ana C. R. D. [UNESP]
dc.contributor.authorMassi, Marcos
dc.contributor.authorSobrinho, Argermiro Soares da Silva
dc.contributor.authorJúnior, Lafayette Nogueira [UNESP]
dc.contributor.institutionInstituto Tecnológico de Aeronáutica (ITA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:06Z
dc.date.available2014-05-27T11:30:06Z
dc.date.issued2013-08-01
dc.description.abstractPurpose: The purpose of this study was to evaluate the effect of diamond-like carbon thin films doped and undoped with silver nanoparticles coating poly(methyl methacrylate) (PMMA) on Candida albicans biofilm formation. The control of biofilm formation is important to prevent oral diseases in denture users. Materials and Methods: Forty-five PMMA disks were obtained, finished, cleaned in an ultrasonic bath, and divided into three groups: Gc, no surface coating (control group); Gdlc, coated with diamond-like carbon film; and Gag, coated with diamond-like carbon film doped with silver nanoparticles. The films were deposited using a reactive magnetron sputtering system (physical vapor deposition process). The specimens were characterized by optical profilometry, atomic force microscopy, and Rutherford backscattering spectroscopy analyses that determined differences in chemical composition and morphological structure. Following sterilization of the specimens by γ-ray irradiation, C. albicans (ATCC 18804) biofilms were formed by immersion in 2 ml of Sabouraud dextrose broth inoculated with a standardized fungal suspension. After 24 hours, the number of colony forming units (cfu) per specimen was counted. Data concerning biofilm formation were analyzed using ANOVA and the Tukey test (p < 0.05). Results: C. albicans biofilm formation was significantly influenced by the films (p < 0.00001), reducing the number of cfu, while not affecting the roughness parameters (p > 0.05). The Tukey test showed no significant difference between Gdlc and Gag. Films deposited were extremely thin (∼50 nm). The silver particles presented a diameter between 60 and 120 nm and regular distribution throughout the film surface (to Gag). Conclusion: Diamond-like carbon films, doped or undoped with silver nanoparticles, coating the base of PMMA-based dentures could be an alternative procedure for preventing candidosis in denture users. © 2013 by the American College of Prosthodontists.en
dc.description.affiliationDepartment of Physics Technological Institute of Aeronautics, São José dos Campos
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis Faculdade de Odontologia de São Jose dos Campos - UNESP - Universidade Estadual Paulista, São José dos Campos
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Faculdade de Odontologia de São Jose dos Campos - UNESP - Universidade Estadual Paulista, São José dos Campos
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis Faculdade de Odontologia de São Jose dos Campos - UNESP - Universidade Estadual Paulista, São José dos Campos
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Faculdade de Odontologia de São Jose dos Campos - UNESP - Universidade Estadual Paulista, São José dos Campos
dc.format.extent451-455
dc.identifierhttp://dx.doi.org/10.1111/jopr.12029
dc.identifier.citationJournal of Prosthodontics, v. 22, n. 6, p. 451-455, 2013.
dc.identifier.doi10.1111/jopr.12029
dc.identifier.issn1059-941X
dc.identifier.issn1532-849X
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.scopus2-s2.0-84881616987
dc.identifier.urihttp://hdl.handle.net/11449/76165
dc.identifier.wosWOS:000323133400005
dc.language.isoeng
dc.relation.ispartofJournal of Prosthodontics
dc.relation.ispartofjcr1.745
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAFM
dc.subjectComplete denture
dc.subjectDenture base
dc.subjectDLC
dc.subjectMagnetron sputtering
dc.subjectNanoparticles
dc.subjectPlasma
dc.subjectProfilometry
dc.subjectRBS
dc.titleEffect of diamond-like carbon thin film coated acrylic resin on candida albicans biofilm formationen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
unesp.author.lattes6543563161403421[3]
unesp.author.orcid0000-0002-2416-2173[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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Artigos - Biociências e Diagnóstico Bucal - ICT
Artigos - Materiais Odontológicos e Prótese - ICT