High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats

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dc.contributor.authorSantos, Caroline Pereira
dc.contributor.authorAguiar, Andreo Fernando
dc.contributor.authorGiometti, Ines Cristina
dc.contributor.authorMariano, Thaoan Bruno
dc.contributor.authorde Freitas, Carlos Eduardo Assumpção
dc.contributor.authorNai, Gisele Alborghetti
dc.contributor.authorde Freitas, Selma Zambelli
dc.contributor.authorPai-Silva, Maeli Dal [UNESP]
dc.contributor.authorPacagnelli, Francis Lopes
dc.contributor.institutionUniversity of Western São Paulo (UNOESTE)
dc.contributor.institutionNorth University of Paraná (UNOPAR)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T16:51:39Z
dc.date.available2018-12-11T16:51:39Z
dc.date.issued2018-05-01
dc.description.abstractThe aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm2, output power 50 mW; energy density 69 J cm−2; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.en
dc.description.affiliationDepartment of Physical Therapy University of Western São Paulo (UNOESTE)
dc.description.affiliationCenter of Research in Health Science North University of Paraná (UNOPAR), Avenue Paris, 675, Jardim Piza
dc.description.affiliationDepartment of Morphology São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Morphology São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 146045/2013-0
dc.format.extent843-850
dc.identifierhttp://dx.doi.org/10.1007/s10103-018-2439-3
dc.identifier.citationLasers in Medical Science, v. 33, n. 4, p. 843-850, 2018.
dc.identifier.doi10.1007/s10103-018-2439-3
dc.identifier.file2-s2.0-85041350074.pdf
dc.identifier.issn1435-604X
dc.identifier.issn0268-8921
dc.identifier.scopus2-s2.0-85041350074
dc.identifier.urihttp://hdl.handle.net/11449/170603
dc.language.isoeng
dc.relation.ispartofLasers in Medical Science
dc.relation.ispartofsjr0,713
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectGallium arsenide laser
dc.subjectIGF-I
dc.subjectMAFbx
dc.subjectMuscle regeneration
dc.subjectMyoD
dc.subjectTNF-α gene expression
dc.titleHigh final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in ratsen
dc.typeArtigo

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