Ocorrência de anticorpos anti-Brucella abortus e anti-Brucella canis em cães rurais e urbanos do Município de Monte Negro, Rondônia, Brasil
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Data
2005-10-01
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Universidade Federal de Santa Maria (UFSM)
Resumo
Foram avaliados 304 cães de ambiente rural e urbano do município de Monte Negro, Rondônia, através do Antígeno Acidificado Tamponado (AAT), Soroaglutinação Lenta em Tubos (SAL) e 2-Mercaptoetanol (2-ME) para a pesquisa de anticorpos anti-Brucella abortus e da Imunodifusão em gel de ágar (IDGA) e Imunodifusão em gel de ágar com soro tratado com 2-Mercaptoetanol (IDGA-ME) para Brucella canis. Foram consideradas positivas as amostras reagentes nas provas confirmatórias do 2-ME e IDGA-ME. Verificaram-se 56 (18,4%) animais reagentes ao AAT e 12 (4,0%) reagentes a SAL. Apenas um cão (0,3%) foi considerado positivo, confirmado pela prova do 2-ME. Foram observadas 11 (3,6%) reações á IDGA, porém não houve confirmação na prova do IDGA-ME. Ressalta-se a baixa ocorrência de cães positivos ao 2-ME e a ausência de animais reagentes á IDGA-ME.
Serum samples from 304 dogs living in urban or rural areas of Monte Negro County, Rondônia State, Brazil, were evaluated for Brucella abortus reactivity using the Rose-Bengal Test (RBT), Standard Tube agglutination Test (SAT), and Mercaptoethanol Test (MET), and for Brucella canis reactivity using the Immunodiffusion test (AGID) and Immunodiffusion test with 2-Mercaptoethanol (ME-AGID). Serum was considered positive if reacted in the MET or ME-AGID. Fifty-six (18.4%) dogs reacted by the RBT, and 12 (4.0%) by the SAT. One (0.3%) dog was positive by the MET. Eleven (3.6%) dogs reacted by the AGID, but with no confirmation by the ME-AGID. It the small occurrence of dogs reacting by the MET and the absence of reactivity by ME-AGID is emphasized.
Serum samples from 304 dogs living in urban or rural areas of Monte Negro County, Rondônia State, Brazil, were evaluated for Brucella abortus reactivity using the Rose-Bengal Test (RBT), Standard Tube agglutination Test (SAT), and Mercaptoethanol Test (MET), and for Brucella canis reactivity using the Immunodiffusion test (AGID) and Immunodiffusion test with 2-Mercaptoethanol (ME-AGID). Serum was considered positive if reacted in the MET or ME-AGID. Fifty-six (18.4%) dogs reacted by the RBT, and 12 (4.0%) by the SAT. One (0.3%) dog was positive by the MET. Eleven (3.6%) dogs reacted by the AGID, but with no confirmation by the ME-AGID. It the small occurrence of dogs reacting by the MET and the absence of reactivity by ME-AGID is emphasized.
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Palavras-chave
Brucella abortus, Brucella canis, cães, Amazônia, sorologia, Brucella abortus, Brucella canis, dogs, Amazon, serology
Como citar
Ciência Rural. Universidade Federal de Santa Maria (UFSM), v. 35, n. 5, p. 1216-1219, 2005.