A Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon)

Página do item simplificado
dc.contributor.authorPinhal, Danillo [UNESP]
dc.contributor.authorShivji, Mahmood S.
dc.contributor.authorNachtigall, Pedro G. [UNESP]
dc.contributor.authorChapman, Demian D.
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionNova SE Univ
dc.contributor.institutionSUNY Stony Brook
dc.date.accessioned2014-05-20T13:50:30Z
dc.date.available2014-05-20T13:50:30Z
dc.date.issued2012-04-09
dc.description.abstractObtaining accurate species-specific landings data is an essential step toward achieving sustainable shark fisheries. Globally distributed sharpnose sharks (genus Rhizoprionodon) exhibit life-history characteristics (rapid growth, early maturity, annual reproduction) that suggests that they could be fished in a sustainable manner assuming an investment in monitoring, assessment and careful management. However, obtaining species-specific landings data for sharpnose sharks is problematic because they are morphologically very similar to one another. Moreover, sharpnose sharks may also be confused with other small sharks (either small species or juveniles of large species) once they are processed (i.e., the head and fins are removed). Here we present a highly streamlined molecular genetics approach based on seven species-specific PCR primers in a multiplex format that can simultaneously discriminate body parts from the seven described sharpnose shark species commonly occurring in coastal fisheries worldwide. The species-specific primers are based on nucleotide sequence differences among species in the nuclear ribosomal internal transcribed spacer 2 locus (ITS2). This approach also distinguishes sharpnose sharks from a wide range of other sharks (52 species) and can therefore assist in the regulation of coastal shark fisheries around the world.en
dc.description.affiliationUNESP São Paulo State Univ, Dept Genet, São Paulo, Brazil
dc.description.affiliationNova SE Univ, Save Our Seas Shark Ctr, Dania, FL USA
dc.description.affiliationNova SE Univ, Oceanog Ctr, Guy Harvey Res Inst, Dania, FL USA
dc.description.affiliationSUNY Stony Brook, Sch Marine & Atmospher Sci, Stony Brook, NY 11794 USA
dc.description.affiliationSUNY Stony Brook, Inst Ocean Conservat Sci, Stony Brook, NY 11794 USA
dc.description.affiliationUNESP São Paulo State Univ, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Genet, São Paulo, Brazil
dc.description.affiliationUnespUNESP São Paulo State Univ, Dept Morphol, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipSave Our Seas Foundation
dc.description.sponsorshipHai Stiftung/Shark Foundation
dc.description.sponsorshipIdFAPESP: 07/03067-8
dc.description.sponsorshipIdFAPESP: 07/03065-5
dc.format.extent6
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0034797
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 7, n. 4, p. 6, 2012.
dc.identifier.doi10.1371/journal.pone.0034797
dc.identifier.fileWOS000305014500032.pdf
dc.identifier.issn1932-6203
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.urihttp://hdl.handle.net/11449/18019
dc.identifier.wosWOS:000305014500032
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPLOS ONE
dc.relation.ispartofjcr2.766
dc.relation.ispartofsjr1,164
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleA Streamlined DNA Tool for Global Identification of Heavily Exploited Coastal Shark Species (Genus Rhizoprionodon)en
dc.typeArtigo
dcterms.licensehttp://www.plos.org/about/open-access/license/
dcterms.rightsHolderPublic Library Science
unesp.author.lattes8858800699425352[5]
unesp.author.orcid0000-0003-3534-974X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000305014500032.pdf
Tamanho:
1.02 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Morfologia - IBB