Severe magnesium deficiency compromises systemic bone mineral density and aggravates inflammatory bone resorption

Página do item simplificado
dc.contributor.authorBelluci, Marina Montosa [UNESP]
dc.contributor.authorde Molon, Rafael Scaf [UNESP]
dc.contributor.authorRossa, Carlos [UNESP]
dc.contributor.authorTetradis, Sotirios
dc.contributor.authorGiro, Gabriela
dc.contributor.authorCerri, Paulo Sergio [UNESP]
dc.contributor.authorMarcantonio, Elcio [UNESP]
dc.contributor.authorOrrico, Silvana Regina Peres [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUCLA School of Dentistry
dc.contributor.institutionUCLA
dc.contributor.institutionGuarulhos University
dc.date.accessioned2020-12-12T01:48:46Z
dc.date.available2020-12-12T01:48:46Z
dc.date.issued2020-03-01
dc.description.abstractWe sought to evaluate the effects of magnesium (Mg) intake deficiency on bone metabolism in rats with induced periodontal disease (PD). Holtzman rats were randomly divided into two groups: Control — animals fed a standard diet and test — animals fed a diet with 90% Mg deficiency. After 60 days on the diets, all animals received ligature on the lower left first molars to induce PD. Animals were euthanized after 30 days following ligature placement. Blood and urine were collected for determination of serum concentrations of Mg, calcium, osteocalcin (OCN), alkaline phosphatase and parathyroid hormone (PTH) by enzyme-linked immunosorbent assay, and the urinary concentration of deoxypyridinoline (DPD). Systemic bone mineral density (BMD), bone volume and architectural bone parameters were evaluated by micro-CT in L4 lumbar vertebrae and mandible. Tartrate-resistant acid phosphatase staining and immunohistochemical (IHC) analysis of inducible nitric oxide synthase (iNOS), Runt-related transcription factor 2 (RUNX2), CD86, CD80, proliferating cell nuclear antigen, vascular endothelial growth factor, OCN and osteopontin were investigated. Reverse-transcription polymerase chain reaction was employed to assess mRNA expression of receptor-activator of nuclear factor-kB ligand, osteoprotegerin (OPG) and interleukin (IL)-6. Mg deficiency was associated with higher concentrations of PTH and DPD, and significant decrease on both systemic and mandibular BMD, as well as greater severity of alveolar and trabecular bone loss. Significant increase in osteoclasts was observed in the test group with PD. IHC analysis showed significant increase in the expression of iNOS and decreased expression of OCN and RUNX2. Increased IL-6 mRNA and decreased OPG mRNA expressions were evidenced in the test group with PD. Mg deficiency caused systemic effects indicative of altered bone metabolism in the vertebrae and affected both immune and stromal cells, aggravating inflammatory bone resorption in the ligature-induced model of periodontitis.en
dc.description.affiliationDepartment of Diagnosis and Surgery Sao Paulo State University School of Dentistry at Araraquara–UNESP
dc.description.affiliationDivision of Diagnostic and Surgical Sciences UCLA School of Dentistry
dc.description.affiliationMolecular Biology Institute UCLA
dc.description.affiliationDepartment of Periodontology and Oral Implantology Dental Research Division Guarulhos University
dc.description.affiliationPhD Department of Morphology Sao Paulo State University School of Dentistry at Araraquara–UNESP
dc.description.affiliationUnespDepartment of Diagnosis and Surgery Sao Paulo State University School of Dentistry at Araraquara–UNESP
dc.description.affiliationUnespPhD Department of Morphology Sao Paulo State University School of Dentistry at Araraquara–UNESP
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2006/05774-0
dc.description.sponsorshipIdFAPESP: 2008/04855-2
dc.description.sponsorshipIdFAPESP: 2015/21697-5
dc.description.sponsorshipIdCAPES: AUXPE 655/2014
dc.identifierhttp://dx.doi.org/10.1016/j.jnutbio.2019.108301
dc.identifier.citationJournal of Nutritional Biochemistry, v. 77.
dc.identifier.doi10.1016/j.jnutbio.2019.108301
dc.identifier.issn1873-4847
dc.identifier.issn0955-2863
dc.identifier.scopus2-s2.0-85076058434
dc.identifier.urihttp://hdl.handle.net/11449/199767
dc.language.isoeng
dc.relation.ispartofJournal of Nutritional Biochemistry
dc.sourceScopus
dc.subjectBone resorption
dc.subjectMagnesium deficiency
dc.subjectOsteoporosis
dc.subjectPeriodontal disease
dc.subjectRats
dc.subjectX-ray microtomography
dc.titleSevere magnesium deficiency compromises systemic bone mineral density and aggravates inflammatory bone resorptionen
dc.typeArtigo
unesp.author.lattes7634063102292261[3]
unesp.author.orcid0000-0003-1705-5481[3]

Arquivos

Coleções

Artigos - Diagnóstico e Cirurgia - FOAR
Artigos - Morfologia - FOAR