Follicular environment as a predictive tool for embryo development and kinetics in cattle

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dc.contributor.authorAlves, Gláucia Pereira
dc.contributor.authorCordeiro, Fernanda Bertuccez
dc.contributor.authorBruna De Lima, Camila
dc.contributor.authorAnnes, Kelly
dc.contributor.authorCristina Dos Santos, Érika
dc.contributor.authorIspada, Jéssica
dc.contributor.authorFontes, Patrícia Kubo [UNESP]
dc.contributor.authorNogueira, Marcelo Fabio Gouveia [UNESP]
dc.contributor.authorNichi, Marcílio
dc.contributor.authorMilazzotto, Marcella Pecora
dc.contributor.institutionFederal University of ABC
dc.contributor.institutionESPOL
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:02:37Z
dc.date.available2019-10-06T16:02:37Z
dc.date.issued2019-01-01
dc.description.abstractFollicular fluid composition and the transcription pattern of granulosa cells were analysed to better comprehend associations between embryo development and morphokinetics. Bovine follicles were punctured and their respective follicular fluid and granulosa cells were collected. Cumulus-oocyte complexes derived from these follicles were matured and fertilised in vitro. Embryo morphology and kinetics were evaluated at 40 h after insemination, when embryos were classified as fast (FCL, four or more cells), slow (SCL, 2-3 cells) or non-cleaved (NCL). Their development was followed until the blastocyst stage. Glucose, pyruvate, cholesterol and oestradiol were quantified in the follicular fluid and the transcription pattern of 96 target genes was evaluated in granulosa cells by large-scale quantitative reverse transcription polymerase chain reaction. Follicular fluid from the blastocyst group had increased levels of glucose, total cholesterol and pyruvate compared to the non-blastocyst group, whereas higher levels of oestradiol were observed in the follicular fluid of embryos and blastocysts with fast cleavage. The transcriptional pattern revealed altered metabolic pathways between groups, such as lipid metabolism, cellular stress and cell signalling. In conclusion, both follicular fluid and granulosa cells are associated with the possibility of identifying follicles that may generate embryos with high potential to properly develop to the blastocyst stage.en
dc.description.affiliationLaboratory of Cellular and Molecular Biology Centre of Natural Sciences and Humanities Federal University of ABC, Av dos Estados, 5005, CEP
dc.description.affiliationLaboratorio para Investigaciones Biomédicas Escuela Superior Politécnica Del Litoral ESPOL
dc.description.affiliationInstitute of Biosciences Universidade Estadual Paulista (UNESP) Campus Botucatu
dc.description.affiliationDepartment of Biological Sciences School of Sciences and Languages Universidade Estadual Paulista (UNESP) Campus Assis
dc.description.affiliationDepartment of Animal Reproduction Faculty of Veterinary Medicine University of São Paulo
dc.description.affiliationUnespInstitute of Biosciences Universidade Estadual Paulista (UNESP) Campus Botucatu
dc.description.affiliationUnespDepartment of Biological Sciences School of Sciences and Languages Universidade Estadual Paulista (UNESP) Campus Assis
dc.format.extent451-461
dc.identifierhttp://dx.doi.org/10.1071/RD18143
dc.identifier.citationReproduction, Fertility and Development, v. 31, n. 3, p. 451-461, 2019.
dc.identifier.doi10.1071/RD18143
dc.identifier.issn1448-5990
dc.identifier.issn1031-3613
dc.identifier.scopus2-s2.0-85055585910
dc.identifier.urihttp://hdl.handle.net/11449/188267
dc.language.isoeng
dc.relation.ispartofReproduction, Fertility and Development
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectbovine
dc.subjectfollicular fluid
dc.subjectgranulosa cells
dc.subjectmorphology
dc.subjectsystem biology
dc.titleFollicular environment as a predictive tool for embryo development and kinetics in cattleen
dc.typeArtigo
unesp.author.orcid0000-0002-5690-9665[2]
unesp.author.orcid0000-0002-4280-9575[7]
unesp.author.orcid0000-0003-4262-0825[9]

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Artigos - Ciências Biológicas - FCLAS