Cyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic rats

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dc.contributor.authorde Lemos Neto, Sylvio Valença [UNESP]
dc.contributor.authorVianna, Isabela Galvão [UNESP]
dc.contributor.authorCastiglia, Yara Marcondes Machado [UNESP]
dc.contributor.authorGolim, Marjorie de Assis [UNESP]
dc.contributor.authorde Souza, Aparecida Vitória Gonçalves [UNESP]
dc.contributor.authorde Carvalho, Lídia Raquel [UNESP]
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authordo Nascimento Junior, Paulo [UNESP]
dc.contributor.authorMódolo, Norma Sueli Pinheiro [UNESP]
dc.contributor.authorVianna, Pedro Thadeu Galvão [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:11:09Z
dc.date.available2018-12-11T17:11:09Z
dc.date.issued2017-03-01
dc.description.abstractPurpose: To investigate the effects of cyclosporine A on renal ischemia-reperfusion injury during transient hyperglycemia in rats. Methods: In a model of ischemia-reperfusion-induced renal injury and transiently induced hyperglycemia by intraperitoneal injection of glucose, 2.5 g.kg-1, Wistar rats were anesthetized with either isoflurane or propofol and received intravenous cyclosporine A, 5 mg.kg-1, five minutes before reperfusion. Comparison groups were isoflurane and propofol sham groups and isoflurane and propofol ischemia-reperfusion-induced renal injury. Renal tubular cell viability was quantitatively assessed by flow cytometry after cell culture and classified as early apoptosis, necrotic cells, and intact cells. Results: Early apoptosis was significantly higher in isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury when compared to both cyclosporine A treated and sham groups. Necrosis percentage was significantly higher in propofol-anesthetized animals subjected to renal ischemia-reperfusion injury. The percentage of intact cells was lower in both, isoflurane and propofol anesthetized animals subjected to renal ischemia-reperfusion injury. Conclusion: In a model of ischemia-reperfusion-induced renal injury, cyclosporine A, 5 m.kg-1, administered five minutes before renal reperfusion in rats with acute-induced hyperglycemia under either isoflurano or propofol anesthesia, attenuated early apoptosis and preserved viability in renal tubular cells, regardless of the anesthetic used.en
dc.description.affiliationDepartment of Anesthesiology Botucatu Medical School Universidade Estadual de São Paulo (UNESP)
dc.description.affiliationDepartment of Anesthesiology Botucatu Medical School UNESP
dc.description.affiliationDepartment of Internal Medicine Botucatu Medical School UNESP
dc.description.affiliationDepartment of Biostatistics Bioscience Institute of Botucatu UNESP
dc.description.affiliationUnespDepartment of Anesthesiology Botucatu Medical School Universidade Estadual de São Paulo (UNESP)
dc.description.affiliationUnespDepartment of Anesthesiology Botucatu Medical School UNESP
dc.description.affiliationUnespDepartment of Internal Medicine Botucatu Medical School UNESP
dc.description.affiliationUnespDepartment of Biostatistics Bioscience Institute of Botucatu UNESP
dc.format.extent203-210
dc.identifierhttp://dx.doi.org/10.1590/S0102-865020170030000004
dc.identifier.citationActa Cirurgica Brasileira, v. 32, n. 3, p. 203-210, 2017.
dc.identifier.doi10.1590/S0102-865020170030000004
dc.identifier.fileS0102-86502017000300203.pdf
dc.identifier.issn1678-2674
dc.identifier.issn0102-8650
dc.identifier.lattes8745358989680600
dc.identifier.orcid0000-0002-2323-9159
dc.identifier.scieloS0102-86502017000300203
dc.identifier.scopus2-s2.0-85017250208
dc.identifier.urihttp://hdl.handle.net/11449/174445
dc.language.isoeng
dc.relation.ispartofActa Cirurgica Brasileira
dc.relation.ispartofsjr0,395
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectApoptosis
dc.subjectCyclosporine
dc.subjectHyperglycemia
dc.subjectKidney
dc.subjectRats
dc.subjectReperfusion injury
dc.titleCyclosporine A attenuates apoptosis and necrosis after ischemia-reperfusion-induced renal injury in transiently hyperglycemic ratsen
dc.typeArtigo
unesp.author.lattes8745358989680600[8]
unesp.author.orcid0000-0002-2323-9159[8]

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