Interactions of indole acetic acid with EGF and FSH in the culture of ovine preantral follicles

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dc.contributor.authorAndrade, E. R.
dc.contributor.authorSeneda, M. M.
dc.contributor.authorAlfieri, A. A.
dc.contributor.authorde Oliveira, J. A.
dc.contributor.authorBracarense, APFRL
dc.contributor.authorFigueiredo, JR
dc.contributor.authorToniolli, R.
dc.contributor.institutionState Univ Ceara
dc.contributor.institutionState Univ Londrina
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:27:20Z
dc.date.available2014-05-20T15:27:20Z
dc.date.issued2005-09-15
dc.description.abstractThe mechanisms that regulate the gradual exit of ovarian follicles from the non-growing, primordial pool are very poorly understood. The objective of this study was to evaluate the effects of adding indole acetic acid (IAA), epidermal growth factor (EGF) and follicle stimulating hormone (FSH) to the media for in vitro culture of ovine ovarian fragments and determine their effects on growth activation and viability of preantral follicles. The ovarian cortex was divided into small fragments; one fragment was immediately fixed in Bouin (control). The other fragments were cultured for 2 or 6 days in culture plates with: minimum essential medium (MEM) supplemented with insulin-transferrin-selenium (ITS), pyruvate, glutamine, hypoxantine, bovine serum albumine and antibiotics (MEM+); MEM+ plus IAA (40 ng/mL); MEM+ plus EGF (100 ng/mL); MEM+ plus FSH (100 ng/mL); MEM+ plus IAA + EGF; MEM+ plus IAA + FSH; MEM+ plus EGF + FSH; or MEM+ plus IAA + EGF + FSH. After 2 or 6 days of culture in each treatment, the pieces of ovarian cortex were fixed in Bonin for histological examination. Follicles were classified as primordial or developing (primary and secondary) follicles. Compared to the control, in all media tested, the percentages of primordial follicles were reduced (P < 0.05) and the percentages of developing follicles were increased (P < 0.05) after 2 or 6 days of culture. Furthermore, culture of ovarian cortex for 6 days reduced the percentages of healthy, viable follicles when compared with the control (P < 0.05), except for cultures supplemented with IAA + EGF and EGF + FSH. In conclusion, the addition of IAA and EGF or EGF and FSH to the culture media were the most effective treatments to sustain the health and viability of activated ovine primordial follicles during in vitro culture. (c) 2005 Elsevier B.V. All rights reserved.en
dc.description.affiliationState Univ Ceara, Fac Vet Med, PPGCV, Fortaleza, Ceara, Brazil
dc.description.affiliationState Univ Londrina, Londrina, PR, Brazil
dc.description.affiliationState Univ São Paulo, Jaboticabal, SP, Brazil
dc.description.affiliationUnespState Univ São Paulo, Jaboticabal, SP, Brazil
dc.format.extent1104-1113
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2005.03.001
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 64, n. 5, p. 1104-1113, 2005.
dc.identifier.doi10.1016/j.theriogenology.2005.03.001
dc.identifier.issn0093-691X
dc.identifier.urihttp://hdl.handle.net/11449/37343
dc.identifier.wosWOS:000231967900006
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectpreantral folliclespt
dc.subjectovinept
dc.subjectIAApt
dc.subjectEGFpt
dc.subjectFSHpt
dc.titleInteractions of indole acetic acid with EGF and FSH in the culture of ovine preantral folliclesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.orcid0000-0002-7024-4487[3]
unesp.author.orcid0000-0002-5097-5119[2]

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