Cytotoxic effects and pulpal response caused by a mineral trioxide aggregate formulation and calcium hydroxide

Página do item simplificado
dc.contributor.authorSouza Costa, Carlos Alberto de Souza [UNESP]
dc.contributor.authorDuarte, Paulo Tobias
dc.contributor.authorSouza, Pedro Paulo Craves de
dc.contributor.authorGiro, Elisa Maria Aparecida
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionSchool of Dentistry
dc.date.accessioned2022-04-29T08:43:55Z
dc.date.accessioned2014-05-20T13:46:09Z
dc.date.available2022-04-29T08:43:55Z
dc.date.available2014-05-20T13:46:09Z
dc.date.issued2008-08-01
dc.description.abstractPurpose: To evaluate the in vivo pulpal response after pulpotomy with different capping agents. In addition, the in vitro cytotoxic effects of both materials were assessed by applying them on culture of pulp cells. Methods: For the in vivo test, the coronal pulp of 28 teeth of dogs was mechanically removed and the root pulps were capped with the following dental materials: Group 1: Pro-Root MTA (PRMTA); and Group 2 (control): calcium hydroxide saline paste (CH). After 60 days, the animals were sacrificed and the teeth processed for histological analysis. In the in vitro test, experimental extracts obtained from both capping agents were applied on the cultured MDPC-23 odontoblast-like cells. Results: In the root pulps capped with PRMTA or CH, coagulation necrosis partially replaced by dystrophic calcification as well as tubular dentin matrix laid down by elongated pulp cells was observed. None or mild inflammatory response occurred beneath the capped pulpal wound. Regarding the pulpal response, PRMTA and CH presented no statistical difference. However, the teeth capped CH presented greater healthy pulp loss which resulted in convex shape of the hard barrier than PRMTA. When applied on the cultured cells, it was demonstrated that PRMTA and CH solutions decreased the cell metabolic activity by 9.9% and 29.4%, respectively. CH caused higher cytotoxic effects to the MDPC-23 cells as well as deeper healthy pulp tissue loss than PRMTA. However, similar sequence of healing occurred after pulpotomy with both dental materials.en
dc.description.affiliationUniversity of São Paulo State/Unesp, Rua Humaitá, 1680, CEP: 14.801-903, CP: 331 - Centro, Araraquara, SP
dc.description.affiliationDepartment of Physiology and Pathology
dc.description.affiliationDepartment of Orthodontics and Pediatric Dentistry School of Dentistry University of São Paulo State, Araraquara, SP
dc.description.affiliationDepartment of Pediatric Dentistry Feira de Santana State University School of Dentistry, Feira de Santana, Bahia
dc.description.affiliationDepartment of Biochemistry Medical School University of São Paulo, Ribeirão Preto, SP
dc.description.affiliationUnespUniversity of São Paulo State/UNESP, Rua Humaitá, 1680, CEP: 14.801-903, CP: 331 - Centro, Araraquara, SP
dc.description.affiliationUnespUniv São Paulo, UNESP, BR-14801903 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 302575/2004-9
dc.format.extent255-261
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/18795523
dc.identifier.citationAmerican Journal of Dentistry, v. 21, n. 4, p. 255-261, 2008.
dc.identifier.citationAmerican Journal of Dentistry. Weston: Mosher & Linder, Inc, v. 21, n. 4, p. 255-261, 2008.
dc.identifier.issn0894-8275
dc.identifier.lattes4517484241515548
dc.identifier.lattes0097031682063652
dc.identifier.scopus2-s2.0-52049109646
dc.identifier.urihttp://hdl.handle.net/11449/243731
dc.identifier.wosWOS:000258968000010
dc.language.isoeng
dc.publisherMosher & Linder, Inc
dc.relation.ispartofAmerican Journal of Dentistry
dc.relation.ispartofjcr0.760
dc.relation.ispartofsjr0,509
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.sourceWeb of Science
dc.titleCytotoxic effects and pulpal response caused by a mineral trioxide aggregate formulation and calcium hydroxideen
dc.typeArtigo
dcterms.rightsHolderMosher & Linder, Inc
unesp.author.lattes4517484241515548[1]
unesp.author.lattes0097031682063652
unesp.author.orcid0000-0002-2085-2951[4]
unesp.author.orcid0000-0001-7266-6061[3]
unesp.author.orcid0000-0002-7455-6867[1]
unesp.author.orcid0000-0003-0707-1912[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

Arquivos

Coleções

Artigos - Clínica Infantil - FOAR