kDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patients

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dc.contributor.authorManoel-Caetano, Fernanda da Silva [UNESP]
dc.contributor.authorAparecida Carareto, Claudia Marcia [UNESP]
dc.contributor.authorBorim, Aldenis Albaneze
dc.contributor.authorMiyazaki, Kenji
dc.contributor.authorSilva, Ana Elizabete [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSch Med
dc.contributor.institutionHosp Base
dc.date.accessioned2014-05-20T14:00:55Z
dc.date.available2014-05-20T14:00:55Z
dc.date.issued2008-11-01
dc.description.abstractTrypanosoma cruzi presents a high degree of intraspecific variability, with possible implications for the pathogenesis of Chagas disease. The aim of this study was to evaluate T cruzi kDNA minicircle gene signatures using the low-stringency single-specific-primer PCR technique in both peripheral blood and oesophageal. mucosa from chronic chagasic patients, with or without megaesophagus, atone or in combination with cardiopathy and megacolon. It was not possible to identify a uniform pattern of shared bands between blood and oesophageal mucosa samples from individuals with the same clinical. form or mixed forms, suggesting multiple T. cruzi infections with differential tissue tropism. Thus, the results indicate that there is an intense intraspecific variability in the hypervariable regions of T cruzi kDNA, which has so far made it impossible to correlate the genetic profile of this structure with the clinical manifestations of Chagas disease. (C) 2008 Royal. Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. AIL rights reserved.en
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationSch Med, FAMERP, Dept Surg, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationHosp Base, Serv Endoscopy, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent1102-1107
dc.identifierhttp://dx.doi.org/10.1016/j.trstmh.2008.05.027
dc.identifier.citationTransactions of The Royal Society of Tropical Medicine and Hygiene. New York: Elsevier B.V., v. 102, n. 11, p. 1102-1107, 2008.
dc.identifier.doi10.1016/j.trstmh.2008.05.027
dc.identifier.issn0035-9203
dc.identifier.urihttp://hdl.handle.net/11449/21526
dc.identifier.wosWOS:000261013500009
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTransactions of the Royal Society of Tropical Medicine and Hygiene
dc.relation.ispartofjcr2.820
dc.relation.ispartofsjr1,174
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectChagas diseaseen
dc.subjectTrypanosoma cruzien
dc.subjectMegaesophagusen
dc.subjectkDNA minicirclesen
dc.subjectGenetic variabilityen
dc.subjectLSSP-PCRen
dc.titlekDNA gene signatures of Trypanosoma cruzi in blood and oesophageal mucosa from chronic chagasic patientsen
dc.typeArtigo
dcterms.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes2503906319038306[5]
unesp.author.orcid0000-0003-1491-8878[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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