Short interspersed CAN SINE elements as prognostic markers in canine mammary neoplasia

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dc.contributor.authorGelaleti, Gabriela B. [UNESP]
dc.contributor.authorGranzotto, Adriana [UNESP]
dc.contributor.authorLeonel, Camila [UNESP]
dc.contributor.authorJardim, Bruna V. [UNESP]
dc.contributor.authorMoschetta, Marina G.
dc.contributor.authorCarareto, Claudia M. A. [UNESP]
dc.contributor.authorZuccari, Debora AP. P. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFaculdade de Medicina de São José do Rio Preto (FAMERP)
dc.date.accessioned2014-12-03T13:11:38Z
dc.date.available2014-12-03T13:11:38Z
dc.date.issued2014-01-01
dc.description.abstractThe genome of mammals is characterized by a large number of non-LTR retrotransposons, and among them, the CAN SINEs are characteristics of the canine species. Small amounts of DNA freely circulate in normal blood serum and high amounts are found in human patients with cancer, characterizing it as a candidate tumor-biomarker. The aim of this study was to estimate, through its absolute expression, the number of copies of CAN SINE sequences present in free circulating DNA of female dogs with mammary cancer, in order to correlate with the clinical and pathological characteristics and the follow-up period. The copy number of CAN SINE sequences was estimated by qPCR in 28 female dogs with mammary neoplasia. The univariate analysis showed an increased number of copies in female dogs with mammary tumor in female dogs >10 years old (p=0.02) and tumor time >18 months (p<0.05). The Kaplan-Meier test demonstrated a negative correlation between an increased number of copies and survival time (p=0.03). High amounts of CAN SINE fragments can be good markers for the detection of tumor DNA in blood and may characterize it as a marker of poor prognosis, being related to female dogs with shorter survival times. This estimate can be used as a prognostic marker in non-invasive breast cancer research and is useful in predicting tumor progression and patient monitoring.en
dc.description.affiliationUNESP Sao Paulo State Univ, Program Postgrad Genet, Sao Paulo, Brazil
dc.description.affiliationFAMERP Fac Med Sao Jose do Rio Preto, Lab Invest Mol Canc, BR-15090000 Sao Paulo, Brazil
dc.description.affiliationUNESP Sao Paulo State Univ, Lab Evolucao Mol Insetos, Sao Paulo, Brazil
dc.description.affiliationFAMERP Fac Med Sao Jose do Rio Preto, Program Postgrad Hlth Sci, BR-15090000 Sao Paulo, Brazil
dc.description.affiliationUNESP Sao Paulo State Univ, Dept Biol, Sao Paulo, Brazil
dc.description.affiliationFAMERP Fac Med Sao Jose do Rio Preto, Dept Mol Biol, BR-15090000 Sao Paulo, Brazil
dc.description.affiliationUnespUNESP Sao Paulo State Univ, Program Postgrad Genet, Sao Paulo, Brazil
dc.description.affiliationUnespUNESP Sao Paulo State Univ, Lab Evolucao Mol Insetos, Sao Paulo, Brazil
dc.description.affiliationUnespUNESP Sao Paulo State Univ, Dept Biol, Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent435-441
dc.identifierhttp://dx.doi.org/10.3892/or.2013.2827
dc.identifier.citationOncology Reports. Athens: Spandidos Publ Ltd, v. 31, n. 1, p. 435-441, 2014.
dc.identifier.doi10.3892/or.2013.2827
dc.identifier.issn1021-335X
dc.identifier.lattes3425772998319216
dc.identifier.orcid0000-0002-0298-1354
dc.identifier.urihttp://hdl.handle.net/11449/113358
dc.identifier.wosWOS:000330788100059
dc.language.isoeng
dc.publisherSpandidos Publ Ltd
dc.relation.ispartofOncology Reports
dc.relation.ispartofjcr2.976
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmammary canceren
dc.subjectcirculating DNAen
dc.subjectCAN SINEen
dc.subjectprognosticen
dc.titleShort interspersed CAN SINE elements as prognostic markers in canine mammary neoplasiaen
dc.typeArtigo
dcterms.rightsHolderSpandidos Publ Ltd
unesp.author.lattes3425772998319216[6]
unesp.author.orcid0000-0002-0298-1354[6]

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais