Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum

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dc.contributor.authorCastro, Karla R.
dc.contributor.authorSetti, Grazielle O. [UNESP]
dc.contributor.authorde Oliveira, Tássia R.
dc.contributor.authorRodrigues-Jesus, Mônica J.
dc.contributor.authorBotosso, Viviane F.
dc.contributor.authorPerini de Araujo, Ana P.
dc.contributor.authorDurigon, Edson L.
dc.contributor.authorFerreira, Luis C.S.
dc.contributor.authorFaria, Ronaldo C.
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionButantan Institute
dc.date.accessioned2023-07-29T13:42:20Z
dc.date.available2023-07-29T13:42:20Z
dc.date.issued2023-05-01
dc.description.abstractZika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV.en
dc.description.affiliationDepartment of Chemistry Federal University of São Carlos, São Carlos
dc.description.affiliationDepartment of Physics School of Technology and Sciences São Paulo State University, Presidente Prudente
dc.description.affiliationInstitute of Biomedical Science University of São Paulo (ICB-USP)
dc.description.affiliationCenter for Development and Innovation Butantan Institute
dc.description.affiliationScientific Platform Pasteur/USP
dc.description.affiliationUnespDepartment of Physics School of Technology and Sciences São Paulo State University, Presidente Prudente
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 157272/2018-4
dc.description.sponsorshipIdFAPESP: 2014/22401-0
dc.description.sponsorshipIdFAPESP: 2016/20045-7
dc.description.sponsorshipIdFAPESP: 2018/04332-1
dc.description.sponsorshipIdCAPES: Finance Code 001
dc.identifierhttp://dx.doi.org/10.1016/j.talanta.2023.124277
dc.identifier.citationTalanta, v. 256.
dc.identifier.doi10.1016/j.talanta.2023.124277
dc.identifier.issn0039-9140
dc.identifier.scopus2-s2.0-85148306927
dc.identifier.urihttp://hdl.handle.net/11449/248376
dc.language.isoeng
dc.relation.ispartofTalanta
dc.sourceScopus
dc.subjectDisposable microfluidic device
dc.subjectElectrochemical immunoassay
dc.subjectMagnetic beads
dc.subjectZika virus infection
dc.titleElectrochemical magneto-immunoassay for detection of zika virus antibody in human serumen
dc.typeArtigo
unesp.author.orcid0000-0002-0579-7667[1]
unesp.author.orcid0000-0002-4883-1693 0000-0002-4883-1693[8]
unesp.author.orcid0000-0003-1094-9597[9]

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