VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors

Página do item simplificado
dc.contributor.authorVilardi, Bruna Maria Rodrigues
dc.contributor.authorBravo-Calderón, Diego Mauricio
dc.contributor.authorBernabé, Daniel Galera [UNESP]
dc.contributor.authorOliveira, Sandra Helena Penha de [UNESP]
dc.contributor.authorOliveira, Denise Tostes
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:17Z
dc.date.available2014-05-27T11:28:17Z
dc.date.issued2013-02-01
dc.description.abstractThe aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 μM) and 1 μM of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production. © 2012 International Society of Oncology and BioMarkers (ISOBM).en
dc.description.affiliationDepartment of Stomatology Bauru School of Dentistry University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, Bauru, 17012-901 São Paulo
dc.description.affiliationDepartment of Pathology and Clinical Propedeutics School of Dentistry of Araçatuba Univ. Estadual Paulista (UNESP), Araçatuba, São Paulo
dc.description.affiliationDepartment of Basic Science School of Dentistry of Araçatuba Univ. Estadual Paulista (UNESP), Araçatuba, São Paulo
dc.description.affiliationUnespDepartment of Pathology and Clinical Propedeutics School of Dentistry of Araçatuba Univ. Estadual Paulista (UNESP), Araçatuba, São Paulo
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry of Araçatuba Univ. Estadual Paulista (UNESP), Araçatuba, São Paulo
dc.format.extent139-143
dc.identifierhttp://dx.doi.org/10.1007/s13277-012-0521-1
dc.identifier.citationTumor Biology, v. 34, n. 1, p. 139-143, 2013.
dc.identifier.doi10.1007/s13277-012-0521-1
dc.identifier.issn1010-4283
dc.identifier.issn1423-0380
dc.identifier.scopus2-s2.0-84872783923
dc.identifier.urihttp://hdl.handle.net/11449/74458
dc.identifier.wosWOS:000313875400018
dc.language.isoeng
dc.relation.ispartofTumor Biology
dc.relation.ispartofsjr1,149
dc.relation.ispartofsjr1,149
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectAdrenergic receptors
dc.subjectOral cancer
dc.subjectVEGF
dc.subjectbeta adrenergic receptor
dc.subjectnoradrenalin
dc.subjectpropranolol
dc.subjectvasculotropin C
dc.subjectcancer cell
dc.subjectcancer cell culture
dc.subjectcell line SCC 25
dc.subjectcell line SCC 9
dc.subjectcontrolled study
dc.subjectenzyme activation
dc.subjectenzyme activity
dc.subjectenzyme synthesis
dc.subjecthuman
dc.subjecthuman cell
dc.subjectin vitro study
dc.subjectmouth squamous cell carcinoma
dc.subjectpriority journal
dc.subjectprotein analysis
dc.subjectprotein expression
dc.subjectprotein function
dc.subjectAdrenergic beta-Antagonists
dc.subjectCarcinoma, Squamous Cell
dc.subjectCell Line, Tumor
dc.subjectHumans
dc.subjectMouth Neoplasms
dc.subjectNeurotransmitter Agents
dc.subjectNorepinephrine
dc.subjectPropranolol
dc.subjectReceptors, Adrenergic, beta
dc.subjectVascular Endothelial Growth Factor C
dc.titleVEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptorsen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights
unesp.author.orcid0000-0003-0805-1120[4]
unesp.author.orcid0000-0002-4628-7129[5]
unesp.author.orcid0000-0002-1225-7749[3]
unesp.author.orcid0000-0003-1813-8051[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

Arquivos

Coleções

Artigos - Patologia e Propedêutica Clínica - FOA
Artigos - Ciências Básicas - FOA