Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring

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dc.contributor.authorYamamoto, Priscila Akemi [UNESP]
dc.contributor.authorBenzi, Jhohann Richard de Lima
dc.contributor.authorde Moraes, Natália Valadares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2021-06-25T10:59:31Z
dc.date.available2021-06-25T10:59:31Z
dc.date.issued2021-01-01
dc.description.abstractAim: We aimed to develop methods to determine gabapentin (GAB) in biological samples using high-performance liquid chromatography (HPLC) with application in pharmacokinetics and therapeutic drug monitoring. Methods: Simple, rapid and efficient HPLC-UV methods to quantify GAB in human plasma and urine were developed and validated for clinical analysis of GAB. The 1-fluoro-2,4-dinitrobenzene (FDNB) was used as derivatization agent. For plasma samples, protein precipitation using acetonitrile was performed, before the derivatization reaction. Urine samples were cleaned-up by liquid-liquid extraction with dichloromethane:n-butanol (1:1, v/v) after derivatized. Amlodipine besilate was used as internal standard (IS). Results: Gabapentin and IS were resolved on LiChrospher® C18 RP column and a mixture of 50 mM sodium phosphate buffer (pH 3.9):methanol (27:73, v/v) as mobile phase, at 1.2 mL/min. The methods used small sample volumes, 100 and 50 µL of plasma and urine, respectively. Linearity was obtained in the interval of 0.2-14 µg/mL in plasma and 2-120 µg/mL in urine. Both methods showed to be selective, without carry-over effect, precise, accurate and stable in different conditions. GAB plasma concentration in patients receiving 600 to 3600 mg/day of GAB ranged between 0.40 to 11.94 µg/mL at steady-state. Conclusions: The methods described in this study were simple, rapid and fulfill all validation requirements. They were easily and successfully applied for population pharmacokinetics and therapeutic drug monitoring of GAB in patients with chronic pain.en
dc.description.affiliationDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.affiliationDepartamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP)
dc.description.affiliationUnespDepartamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2015/25728-2
dc.identifierhttp://dx.doi.org/10.4322/2179-443X.0717
dc.identifier.citationRevista de Ciencias Farmaceuticas Basica e Aplicada, v. 42.
dc.identifier.doi10.4322/2179-443X.0717
dc.identifier.issn2179-443X
dc.identifier.issn1808-4532
dc.identifier.scopus2-s2.0-85105390895
dc.identifier.urihttp://hdl.handle.net/11449/207701
dc.language.isoeng
dc.relation.ispartofRevista de Ciencias Farmaceuticas Basica e Aplicada
dc.sourceScopus
dc.subjectGabapentin
dc.subjectHPLC
dc.subjectPlasma
dc.subjectUrine
dc.titleSimple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoringen
dc.typeArtigo

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