Morphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryos

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dc.contributor.authorMacHado, Grazieli M.
dc.contributor.authorFerreira, Allice R. [UNESP]
dc.contributor.authorGuardieiro, Monique M.
dc.contributor.authorBastos, Michele R. [UNESP]
dc.contributor.authorCarvalho, José O.
dc.contributor.authorLucci, Carolina M.
dc.contributor.authorDiesel, Tiago O.
dc.contributor.authorSartori, Roberto
dc.contributor.authorRumpf, Rodolfo
dc.contributor.authorFranco, Maurício M.
dc.contributor.authorDode, Margot A. N.
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.institutionUniversity of Brasília
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:29:00Z
dc.date.available2014-05-27T11:29:00Z
dc.date.issued2013-04-29
dc.description.abstractThe present study was designed to compare Day 14 bovine embryos that were produced entirely in vitro using the post-hatching development (PHD) system with in vivo-derived embryos without or with transient PHD culture from Day 7 to Day 14. Embryos on Day 14 were used for sex determination and gene expression analysis of PLAC8, KRT8, CD9, SLC2A1, SLC2A3, PGK1, HSF1, MNSOD, HSP70 and IFNT using real-time quantitative (q) polymerase chain reaction (PCR). First, Day 7 in vivo-and in vitro-produced embryos were subjected to the PHD system. A higher rate of survival was observed for in vitro embryos on Day 14. Comparing Day 14 embryos produced completely in vivo or completely in vitro revealed that the mean size of the former group was greater than that of the latter (10.29±1.83 vs 2.68±0.33mm, respectively). Expression of the HSP70 and SLC2A1 genes was down-and upregulated, respectively, in the in vitro embryos. The present study shows that in vitro embryos cultured in the PHD system are smaller than in vivo embryos and that of the 10 genes analysed, only two were differentially expressed between the two groups. These findings indicate that, owing to the poor survival rate, the PHD system is not reliable for evaluation of in vitro embryo quality. © 2013 CSIRO.en
dc.description.affiliationLaboratory of Animal Reproduction Embrapa Genetic Resources and Biotechnology Parque Estação Biológica, Av. W5 Norte (final), Caixa Postal 02372, Brasília, DF 70770-917
dc.description.affiliationCollege of Agriculture and Veterinary University of Brasília Campus Universitário Darcy Ribeiro, Caixa Postal 4508, Brasília, DF 70910-970
dc.description.affiliationCollege of Veterinary and Animal Science São Paulo State University, Distrito Rubião Jú nior, Botucatu, SP 18618-970
dc.description.affiliationDepartment of Animal Science College of Agriculture Luiz de Queiroz (ESALQ) University of São Paulo, Piracicaba, SP 13418-900
dc.description.affiliationDepartment of Physiological Science Institue of Biological Science University of Brasília, Brasília, DF 70910-970
dc.description.affiliationUnespCollege of Veterinary and Animal Science São Paulo State University, Distrito Rubião Jú nior, Botucatu, SP 18618-970
dc.format.extent600-608
dc.identifierhttp://dx.doi.org/10.1071/RD11282
dc.identifier.citationReproduction, Fertility and Development, v. 25, n. 4, p. 600-608, 2013.
dc.identifier.doi10.1071/RD11282
dc.identifier.issn1031-3613
dc.identifier.issn1448-5990
dc.identifier.scopus2-s2.0-84876537696
dc.identifier.urihttp://hdl.handle.net/11449/75179
dc.identifier.wosWOS:000317672900002
dc.language.isoeng
dc.relation.ispartofReproduction, Fertility and Development
dc.relation.ispartofjcr2.105
dc.relation.ispartofsjr0,681
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectagarose tunnels
dc.subjectpost-hatching embryos
dc.subjectanimal experiment
dc.subjectcow
dc.subjectdown regulation
dc.subjectembryo
dc.subjectfemale
dc.subjectgene expression
dc.subjectin vitro study
dc.subjectin vivo study
dc.subjectmorphology
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectsex determination
dc.subjectsex ratio
dc.subjectsurvival rate
dc.subjectupregulation
dc.titleMorphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryosen
dc.typeArtigo
dcterms.licensehttp://www.publish.csiro.au/media/client/CopyrightAssign.pdf

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